摘要：腸病毒 71 型為可引起致命及重症的腸病毒，尤其是在台灣、中國大陸以及東南亞。另一個重要且有高度傳染力的病毒則是腺病毒，可引起幼童有重症或是致死的案例。然而目前沒有針對腸病毒71 型和腺病毒感染的抗病毒治療藥物。我們在第一年的前半年篩選對抗腸病毒71 型和腺病毒的中草藥。我們篩選多種不同萃取方式的中草藥萃取物，藉由病毒斑(plaque assay)和細胞毒殺試驗(cytotoxicity assay)測定其IC50(50%抑制病毒濃度)，以及CC50(50%細胞毒性濃度)以計算出選擇指數(selective index, SI)。根據IC50 及選擇指數，我們已選出幾個具有潛力的植物藥萃取物(如EPA-W, EPA-E, SMf-2-E及 SMd-E 對腺病毒有相當低的IC50: 約4 至10 ug/ml,且無明顯細胞毒性, SI>300; 而SMf-2-E2對腸病毒71 型有相當低的IC50: 約8 ug/ml) ，將做進一步探討其抗病毒作用機制。目前已測試EPA-W 發現其抗腺病毒的機轉主要為抑制病毒其病毒貼附、部分為抑制病毒進入細胞。而有一個深具潛力之植物藥正進行其前期專利之申請。第二及第三年度將繼續篩選更多有潛力之中草藥/植物藥，有效者更進一步研究其抗病毒作用機制。我們希望本研究可以發掘新奇具有抗病毒潛力的藥物，可以幫助預防或是治療重要、具有高致死率並且目前尚未有效抗病毒藥物的腸病毒71 型和腺病毒感染，未來可進一步進行產學合作及臨床試驗以確認其療效。
Abstract: There were large outbreaks of enterovirus 71 (EV71) infections resulting in dozens of deaths inBulgaria in 1975, Hungary in 1978, and Malaysia in 1997, Taiwan since 1998 and mainland Chinasince 2007. EV71 may become the most important enterovirus related to the fatal and morbid cases,especially in Taiwan, China and the southeast Asia. The other important and highly transmissiblevirus is adenovirus which may cause complications or hospitalization, and some severe or fatal casesin young children. However, there is no antiviral therapy for EV71 and adenovirus infection.Traditional Chinese medicine and herbs have shown strong potential because of multi-targetedmechanisms of action, fewer side effects and possible less drug resistance.In the first half year of this study, we screened a lot of herbs against EV71 and adenoviruses.We screened antiviral activities of herbs including extraction with different methods and defined theIC50 (50% inhibitory concentration) and CC50 (50% cytotoxicity concentration) by plaque assay andcytotoxicity assay to calculate selective index (SI) for EV71 and adenovirus. According to IC50 andSI, some potential candidates (EPA-W, EPA-E, SMf-2-E and SMd-E for adenovirus with IC50 of 3-10ug/ml and SI>300; SMf-2-E2 for EV71 with IC50 8 ug/ml) were chosen to further investigate theirantiviral mechanism including attachment, entry, replication, and viral release. We found that EPA-Wcan inhibit mainly viral attachment and somewhat viral entry. The most potential herb is now on theprocess for patent provisional application.We will continue to screen and find effective herbs against important viruses and investigatetheir antiviral mechanism in the second and third years. We hope that this study can discover novelpotential antiviral drugs to do further clinical trials and establish the further prevention or treatmentfor EV71 and adenovirus, which are important, potentially fatal and currently without effectiveantiviral drugs.