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https://scholars.lib.ntu.edu.tw/handle/123456789/37124
2024-03-28T17:46:19ZInteraction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments
https://scholars.lib.ntu.edu.tw/handle/123456789/641111
標題: Interaction of vascular endothelial cells with hydrophilic fullerene nanoarchitectured structures in 2D and 3D environments
作者: Chen, Tsai Yu; Cheng, Kun Chih; Yang, Pei Syuan; Shrestha, Lok Kumar; Ariga, Katsuhiko; SHAN-HUI HSU
摘要: The interaction between diverse nanoarchitectured fullerenes and cells is crucial for biomedical applications. Here, we detailed the preparation of hydrophilic self-assembled fullerenes by the liquid-liquid interfacial precipitation (LLIP) method and hydrophilic coating of the materials as a possible vascularization strategy. The interactions of vascular endothelial cells (ECs) with hydrophilic fullerene nanotubes (FNT-P) and hydrophilic fullerene nanowhiskers (FNW-P) were investigated. The average length and diameter of FNT-P were 16 ± 2 μm and 3.4 ± 0.4 μm (i.e. aspect ratios of 4.6), respectively. The average length and diameter of FNW-P were 65 ± 8 μm and 1.2 ± 0.2 μm (i.e. aspect ratios of 53.9), respectively. For two-dimensional (2D) culture after 7 days, the ECs remained viable and proliferated up to ~ 420% and ~ 400% with FNT-P and FNW-P of 50 μg/mL, respectively. Furthermore, an optimized chitosan-based self-healing hydrogel with a modulus of ~400 Pa was developed and used to incorporate self-assembled fullerenes as in vitro three-dimensional (3D) platforms to investigate the impact of FNT-P and FNW-P on ECs within a 3D environment. The addition of FNW-P or FNT-P (50 μg/mL) in the hydrogel system led to proliferation rates of ECs up to ~323% and ~280%, respectively, after 7 days of culture. The ECs in FNW-P hydrogel displayed an elongated shape with aligned morphology, while those in FNT-P hydrogel exhibited a rounded and clustered distribution. Vascular-related gene expressions of ECs were significantly upregulated through interactions with these fullerenes. Thus, the combined use of different nanoarchitectured self-assembled fullerenes and self-healing hydrogels may offer environmental cues influencing EC development in a 3D biomimetic microenvironment, holding promise for advancing vascularization strategy in tissue engineering.2024-01-01T00:00:00ZOptimizing Emission Stability in Blue Perovskite Light-Emitting Diodes via Oxygen-Plasma Treatment of NixOy Hole Transport Layer
https://scholars.lib.ntu.edu.tw/handle/123456789/640169
標題: Optimizing Emission Stability in Blue Perovskite Light-Emitting Diodes via Oxygen-Plasma Treatment of NixOy Hole Transport Layer
作者: Yan, Zhen Li; Huang, Hong Yu; Benas, Jean Sebastien; Yang, Ching Wei; Su, Chun Jen; Liang, Fang Cheng; Chen, Wei Cheng; Tsai, Hsinhan; RU-JONG JENG; Kuo, Chi Ching
摘要: Currently, wide-color gamut perovskite light-emitting diodes (PeLEDs) are commonly controlled through halide composition tuning. However, the formation of perovskite crystals involving different ion radii of halogens often results in structural fragility and ionic defects, leading to emission instability during operation. This study showcases a novel approach to achieving a homogeneous and low-defect crystalline state by regulating the thermodynamic nucleation mechanism of sky-blue perovskite, thereby enhancing emission stability. Utilizing oxygen-plasma treatment, a highly uniform surface energy is ensured for the nickel oxide acting as a hole transport layer. This treatment not only induces homogeneous nucleation of the perovskite layer but also effectively suppresses crystal defects. Simultaneously, the uniform surface energy alleviates halide phase separation caused by Ostwald ripening. Upon optimizing the fabrication conditions for sky-blue perovskite, the resulting PeLEDs, featuring an electroluminescent peak at 486 nm, attain a luminance of 1125.3 cd m−2 with exceptional electroluminescent stability and a T50 lifetime. Furthermore, gaining insights into the thermodynamic nucleation mechanism in mix-halides and perovskites contributes to the advancement of research on sky-blue PeLEDs and offers valuable perspectives for future development.2024-01-01T00:00:00ZLuminescent lanthanide-containing gelatin/polydextran/laponite nanocomposite double-network hydrogels for processing and sensing applications
https://scholars.lib.ntu.edu.tw/handle/123456789/640168
標題: Luminescent lanthanide-containing gelatin/polydextran/laponite nanocomposite double-network hydrogels for processing and sensing applications
作者: Chiang, Pei Yu; Zeng, Pin Han; YI-CHEUN YEH
摘要: Lanthanide-containing nanomaterials have gained significant popularity for their utilization in polymeric networks, enabling the creation of luminescent nanocomposites for advanced applications. In this study, we developed a new type of lanthanide-containing nanocomposite hydrogels by incorporating terbium-containing laponite (Tb3+@Lap) into the networks of polyethyleneimine-modified gelatin/polydextran aldehyde (PG/PDA) through dynamic bonds. The structures and properties of the Tb3+@Lap-containing nanocomposite double-network (ncDN) hydrogels were comprehensively investigated in comparison with the DN hydrogels with a pure polymeric network and the Lap-containing ncDN hydrogels. The PG/PDA/Tb3+@Lap ncDN hydrogels with multiple dynamic bonds (i.e., imine bonds, coordination bonds, hydrogen bonds, and electrostatic interactions) exhibited remarkable characteristics of shear-thinning and self-healing, making them suitable for the construction of hydrogel scaffolds on a macroscale using fabrication techniques such as electrospinning and 3D printing. Moreover, the PG/PDA/Tb3+@Lap ncDN hydrogels have been demonstrated to act as sensitive and selective luminescent sensors for detecting copper ions. Taken together, a versatile lanthanide-containing ncDN hydrogel platform capable of dynamic features is developed for processing and sensing applications.2024-03-01T00:00:00ZSelf-Healing Hydrogel Containing Decellularized Liver Matrix and Endothelial Cell-Covered Hepatocyte Spheroids for Rescue of Injured Hepatocytes
https://scholars.lib.ntu.edu.tw/handle/123456789/640167
標題: Self-Healing Hydrogel Containing Decellularized Liver Matrix and Endothelial Cell-Covered Hepatocyte Spheroids for Rescue of Injured Hepatocytes
作者: Chou, Xin Yu; Cheng, Kai Yi; Yin, Wei Rong; Cheng, Tzong-Jih; Chen, Richie L. C.; SHAN-HUI HSU; YUNG-TE HOU
摘要: Liver fibrosis occurs in many chronic liver diseases, while severe fibrosis can lead to liver failure. A chitosan-phenol based self-healing hydrogel (CP) integrated with decellularized liver matrix (DLM) is proposed in this study as a 3D gel matrix to carry hepatocytes for possible therapy of liver fibrosis. To mimic the physiological liver microenvironment, DLM is extracted from pigs and mixed with CP hydrogel to generate DLM-CP self-healing hydrogel. Hepatocyte spheroids coated with endothelial cells (ECs) are fabricated using a customized method and embedded in the hydrogel. Hepatocytes injured by exposure to CCl4-containing medium are used as the in vitro toxin-mediated liver fibrosis model, where the EC-covered hepatocyte spheroids embedded in the hydrogel are co-cultured with the injured hepatocytes. The urea synthesis of the injured hepatocytes reaches 91% of the normal level after 7 days of co-culture, indicating that the hepatic function of injured hepatocytes is rescued by the hybrid spheroid-laden DLM-CP hydrogel. Moreover, the relative lactate dehydrogenase activity of the injured hepatocytes is decreased 49% by the hybrid spheroid-laden DLM-CP hydrogel after 7 days of co-culture, suggesting reduced damage in the injured hepatocytes. The combination of hepatocyte/EC hybrid spheroids and DLM-CP hydrogel presents a promising therapeutic strategy for hepatic fibrosis.2024-01-01T00:00:00Z