https://scholars.lib.ntu.edu.tw/handle/123456789/110897
標題: | 個體特性對攝食含有機砷海產食物後尿中砷物種分佈之影響 | 作者: | 黃耀輝 | 關鍵字: | 有機砷;無機砷;牡蠣;液相層 析串聯感應耦合電漿質譜儀;砷代謝物種;Organic Arsenic;Inorganic Arsenic;Oyster;HPLC-ICP-MS;Arsenic Metabolites | 公開日期: | 31-七月-2004 | 出版社: | 臺北市:國立臺灣大學公共衛生學院職業醫學與工業衛生研究所 | 摘要: | 本計畫探討個體特性對被攝食到人體 內之海產中有機砷代謝物種分佈的影響, 以及不同海產食物有機砷攝食量下對尿中 總無機砷代謝物種含量所造成的干擾程 度。本次研究共徵求38 名未受砷暴露之健 康者進行七天半飲食控制實驗,在第四天 晚餐提供49.9 ±7.4 g 牡蠣作為有機砷暴露 來源。實驗期間第一至第三天收集早晨初 尿,之後四天半收集早晨初尿和傍晚各一 次尿液來觀察尿中砷代謝的趨勢。最後有 21 名受試者提供全部完整的尿液樣本進行 分析。牡蠣樣本與尿液樣本均以感應耦合 電漿質譜儀(ICP-MS)量測總砷量,另以高 效能液相層析儀(HPLC )串聯感應耦合電 漿質譜儀進行分析As III 、As V 、MMA 、 DMA 、arsenobetaine (AsBe )、arsenocholine (AsCho )、trimethylarsine oxide (TMAO )、 tetramethylarsonium ion (TetMA )等八種砷 物種。結果顯示,攝食牡蠣中有機砷後, 尿中代謝物以DMA 和AsBe 為主,還有少 量As III 、MMA 、TMAO 和TetMA ,以及 一個未知的砷物種,As V 和AsCho 則較少 在尿中被偵測到。經過控制單位體重牡蠣 攝食量的影響後,尿中總砷和AsBe 的濃度 與性別有關。同時,受試者間對於DMA 及AsBe 的吸收代謝能力有個體間差異存 在。Mixed Effect Model 分析結果顯示,受 試者攝食牡蠣後各日尿中各種砷物種濃度 均有明顯上升。此外,應用此模式係數可 進一步預測一般受試者食入定量牡蠣後尿 中DMA 、AsBe 及總砷濃度的變化趨勢, 以評估其對無機砷暴露後尿中砷代謝物種 的干擾程度。 The aims of the present study were to explore the effects of inter-individual variability on the distribution of urinary organic arsenic metabolites, and to what extent the interference resulting from the intake of seafood organic arsenic on the urinary inorganic arsenic metabolites. Totally 38 healthy volunteers without arsenic exposure were recruited in this seven-and-half-day diet control study, and were served 49.9 ±7.4 g oyster at the fourth day’s dinner as organic arsenic exposure source. First morning urine void were collected during the whole study period while after-shift urine void were collected around dusk of the last 4 days. Finally, 21 participants with complete urine samples were included for further urinary arsenic determination and data analysis. Both oyster and urine samples were analyzed with inductive coupled plasma – mass spectrometry for total arsenic level, and for As III , As V , MMA, DMA, arsenobetaine (AsBe), arsenocholine (AsCho), trimethylarsine oxide (TMAO), tetramethylarsonium ion (TetMA) by linked with high performance liquid chromatography. Results showed that, after ingesting organic arsenic in oyster, urinary arsenic species were dominated by DMA and AsBe, while less amounts were found for As III , MMA, TMAO, TetMA, and an unknown arsenic species. As V and AsCho were seldom detected compared to others. After controlling the oyster intake per unit body weight, gender was shown associated with the urinary total arsenic and AsBe content in urine. Meanwhile, individual variability was demonstrated among the study participants for the DMA and AsBe metabolism. Mixed effect model analysis illustrated that various urinary arsenic metabolites determined in the present study were elevated significantly after oyster ingestion. Besides, using the derived mixed effect model, it is possible to predict the urinary DMA, AsBe and total arsenic levels of a subject ingesting a certain amount of oyster, and the extent it may interfere with the urinary inorganic arsenic metabolites assessment. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/4974 | 其他識別: | 922320B002169 | Rights: | 國立臺灣大學公共衛生學院職業醫學與工業衛生研究所 |
顯示於: | 環境與職業健康科學研究所 |
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