https://scholars.lib.ntu.edu.tw/handle/123456789/111145
標題: | EFFECTS ON SISTER CHROMATID EXCHANGE FREQUENCY OF POLYMORPHISMS IN DNA REPAIR GENE XRCC1 IN SMOKERS | 作者: | LEI, YU-CHEN HWANG, SHIN-JEN CHANG, CHUN-CHAO KUO, HSIEN-WEN LUO, JIIN-CHYUAN CHANG, MING, J., W. CHENG, TSUN-JEN |
關鍵字: | microsomal epoxide hydrolase;X-ray cross-complementing group 1;glutathione S-transferase M1;sister chromatid exchange;cigarette smoking;polymorphism | 公開日期: | 2002 | 卷: | v.519 | 期: | n.1-2 | 起(迄)頁: | 93-101 | 來源出版物: | MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 摘要: | The association between metabolic polymorphisms and cigarette smoking-induced cancers has been documented. However, the role of DNA repair polymorphism in carcinogenesis is less clear. To investigate if the polymorphisms of metabolic traits and DNA repair modulate smoking-related DNA damage, we used sister chromatid exchange (SCE) as a marker of genetic damage to explore the relationship of microsomal epoxide hydrolase (mEH), glutathione S-transferase M1 (GSTM1), and X-ray cross- complementing group 1 (XRCC1) and cigarette smoking-induced SCE. Sixty-one workers without significant exposure to mutagens were recruited. Questionnaires were completed to obtain detailed occupational, smoking, and medical histories . SCE frequency in peripheral lymphocytes was determined using a standard cytogenetic assay and GSTM1, mEH (exons 3 and 4), XRCC1 (codon 399) genotypes were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR/PFLP). Smokers had higher SCE frequency than non-smokers (8.4 versus 7.1, P < 0.05). Among workers who had smoked equal to or greater than 10 cigarettes each day, those with XRCC1 Arg/Gln + Gln/Gln had higher SCE frequency than those with XRCC1 Arg/Arg after adjusting for potential confounders (9.0 versus 7.9, P < 0.05). The interaction of XRCC1 and cigarettes smoked per day on SCE frequency was also observed (P = 0.02). There was no significant interaction between cigarettes smoked per day with GSTM1 and mEH on SCE frequency. Our results support previous epidemiological studies that XRCC1 may play a role in cigarette smoking-induced lung cancer. (C) 2002 Elsevier Science B.V. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/80250 |
顯示於: | 環境與職業健康科學研究所 |
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