https://scholars.lib.ntu.edu.tw/handle/123456789/111853
標題: | Zinc Oxide Particles Induce Inflammatory Responses in Vascular Endothelial Cells Via Nf-Kappa B Signaling | 作者: | TSOU, TSUI-CHUN YEH, SZU-CHING TSAI, FENG-YUAN LIN, HO-JANE CHENG, TSUN-JEN |
關鍵字: | Zinc oxide;Endothelium;Inflammation;NF-kappa B;Adhesion molecules;Cytokines | 公開日期: | 2010 | 卷: | v.183 | 期: | n.1-3 | 起(迄)頁: | 182-188 | 來源出版物: | JOURNAL OF HAZARDOUS MATERIALS | 摘要: | This study investigated inflammatory effects of zinc oxide ( ZnO) particles on vascular endothelial cells. The effects of 50 and 100-nm ZnO particles on human umbilical vein endothelial cells (HUVECs) were characterized by assaying cytotoxicity, cell proliferation, and glutathione levels. A marked drop in survival rate was observed when ZnO concentration was increased to 45 mu g/ml. ZnO concentrations of <= 3 mu g /ml resulted in increased cell proliferation, while those of <= 45 mu g/ml caused dose- dependent increases in oxidized glutathione levels. Treatments with ZnO concentrations <= 45 mu g/ml were performed to determine the expression of intercellular adhesion molecule-1 (ICAM-1) protein, an indicator of vascular endothelium inflammation, revealing that ZnO particles induced a dose-dependent increase in ICAM-1 expression and marked increases in NF-kappa B reporter activity. Overexpression of I kappa B alpha completely inhibited ZnO-induced ICAM-1 expression, suggesting NF-kappa B plays a pivotal role in regulation of ZnO-induced inflammation in HUVECs. Additionally. TNF-alpha, a typical inflammatory cytokine, induced ICAM-1 expression in an NF- kappa B-dependent manner, and ZnO synergistically enhanced TNF-alpha-induced ICAM-1 expression. Both 50 and 100-nm ZnO particles agglomerated to similar size distributions. This study reveals an important role for ZnO in modulating inflammatory responses of vascular endothelial cells via NF- kappa B signaling, which could have important implications for treatments of vascular disease. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/235898 |
顯示於: | 環境與職業健康科學研究所 |
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