https://scholars.lib.ntu.edu.tw/handle/123456789/112662
標題: | DIFFERENCES IN REPLICATION CAPACITY BETWEEN ENTEROVIRUS 71 ISOLATES OBTAINED FROM PATIENTS WITH ENCEPHALITIS AND THOSE OBTAINED FROM PATIENTS WITH HERPANGINA IN TAIWAN | 作者: | KUNG, CHIEN-MIN KING, CHWAN-CHUEN LEE, CHUN-NAN HUANG, LI-MIN |
關鍵字: | enterovirus 71;virus variation;cellular tropism | 公開日期: | 2007 | 卷: | VIROLOGY | 期: | n.1 | 起(迄)頁: | 60-68 | 來源出版物: | JOURNAL OF MEDICAL | 摘要: | The cellular-tropism and biological characteristics of enterovirus 71 (EV71) isolates in Taiwan (TW) were studied. Growth curve experiments were conducted using cell lines that were possibly exhibited pathogenesis, and RT-PCR and sequencing tests were undertaken to amplify the 5' non- coding region (5'-NCR). The encephalitis isolate EV71 TW98 NTU2078 was PBMCtropic, temperature-resistant (Tr) at 40 degrees C, and easier to replicate in HTB-14 (astrocytoma) than the herpangina isolate EV71 TW98NTU 1186 (The viral yields were 100-fold higher than those of the herpangina isolate EV71 TW98NTU 1186 at 96 hr post infection.). The herpangina isolate EV71 TW98NTU 1186 was non-PBMC-tropic, and temperature-sensitive (Ts) at 40 degrees C. The replication of EV71 TW98NTU1186 in HTB-14 was lower. No EV71 isolate infected HTB-37 (human colon adenocarcinoma cells). The encephalitis EV71 isolate exhibited better replication and transmission in PBMCs and astrocytes than did the EV71 isolate without CNS involvement. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/82086 |
顯示於: | 流行病學與預防醫學研究所 |
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