https://scholars.lib.ntu.edu.tw/handle/123456789/114580
標題: | OXIDATIVE DNA DAMAGE ESTIMATED IN RELATION TO INDOOR CARBON DIOXIDE, VOLATILE ORGANICS AND TOBACCO SMOKE EXPOSURES | 作者: | MA, YEE-CHUNG LIN, JIA-MING SUNG, FUNG-CHANG |
公開日期: | 2005 | 卷: | v.16 | 期: | n.5 | 起(迄)頁: | S4-2 | 來源出版物: | EPIDEMIOLOGY | 摘要: | Introduction: Carbon dioxide (CO2), volatile organic compounds (VOCs) and environmental tobacco smoke (ETS) are common indoor air pollutants generated from human activities in buildings. This study investigated urinary 8- hydroxydeoxyguanosine (8-OHdG) as an effective biomarker of DNA damage associated with indoor air pollutants among office building occupants. Methods: With consents and indoor CO2 and VOCs measured simultaneously, urinary 8-OHdG and cotinine were determined for 344 non-smokers and 45 smokers recruited from 8 air-conditioned office buildings in Taipei. CO2and total VOCs were measured with portable monitors (Q- TRAK IAQ Model 8551, TSI Incorporated, Shoreview, MN, U.S.A, and PGM-7240, RAE SYSTEMS, California, U.S.A., respectively ). Urinary 8-OHdG and cotinine were determined by enzyme- linked immunosorbent assay (ELISA) and barbituric acid assay , respectively. Results: There were large variations in concentrations of CO2 (median= 901 ppm; range=“530~2794” ppm) and total VOCs (median= 190 ppb; range= 1~55733 ppb), and urinary cotinine levels (median= 2135 mg/g creatinine; range= 486~8984 mg/g creatinine). The average 8-OHdG levels were approximately two times higher in smokers than in non- smokers for both females (5.06 vs. 11.3 mg/g creatinine) and males (5.08 vs.10.6 mg/g creatinine). The multivariate logistic regression analysis showed that, comparing with individuals with CO2 exposure at the lowest quartile, the odds ratios (ORs) of having the urinary 8-OHdG levels greater than the overall median value, 4.99 mg/g creatinine, were 7.66 (95% confidence interval (CI) = 3.08–19.1) for individuals with the exposure at the third quartile level, and 14.3 (95% CI = 14.13–49.7) for those at the highest quartile level. Smokers were 6.33 times (95% CI = 1.44–27.8 ) more likely than never smokers to have such an elevated urinary 8-OHdG level. There was no significant association between VOCs and 8-OHdG excreted. Conclusion: The indoor CO2 and tobacco smoke exposure contribute independently to elevated DNA damage, using urinary 8-OHdG levels as a sensitive biomarker. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/81781 |
顯示於: | 環境與職業健康科學研究所 |
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