https://scholars.lib.ntu.edu.tw/handle/123456789/136521
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Chi-Iou | en |
dc.contributor.author | Hsieh, Chun-Hung | en |
dc.contributor.author | Lee, Stanley Shiao-Ying | en |
dc.contributor.author | Lee, Wei-Shan | en |
dc.contributor.author | Chang-Chien, Gou-Ping | en |
dc.contributor.author | Pan, Chien-Yuan | en |
dc.contributor.author | Lee, Hsinyu | en |
dc.creator | Lin, Chi-Iou; Hsieh, Chun-Hung; Lee, Stanley Shiao-Ying; Lee, Wei-Shan; Chang-Chien, Gou-Ping; Pan, Chien-Yuan; Lee, Hsinyu | - |
dc.date | 2008 | en |
dc.date.accessioned | 2009-06-24T06:12:54Z | - |
dc.date.accessioned | 2018-07-06T01:31:17Z | - |
dc.date.available | 2009-06-24T06:12:54Z | - |
dc.date.available | 2018-07-06T01:31:17Z | - |
dc.date.issued | 2008 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/161584 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw/bitstream/246246/161584/1/31.pdf | - |
dc.description.abstract | Dioxins comprise a group of compounds which contain a double aromatic ring-like structure. They are among the most prevalent and toxic environmental pollutants. Accumulation of dioxins in human tissues poses a potential threat to human health. Currently, analytical chemical procedures dominate dioxin-detection protocols. In this study, we established a fluorescence resonance energy transfer (FRET)-based dioxin-detection bioassay. Aryl hydrocarbon receptor (AHR) and AHR nuclear translocator (ARNT) fused-cyan fluorescent protein (CFP) and -yellow fluorescent protein (YFP) constructed were transiently co-transfected into rat hepatoma cell line, H4IIEC3 cells. Our results showed that no FRET signals were detected in AHR-CFP- and ARNT-YFP-transfected H4IIEC3 cells. However, dioxin treatments upregulated FRET signals in these transfected cells in a dose-dependent manner. This work highlighted the potential of FRET technique in the detection of dioxin-like compounds. ? 2008 National Science Council Taipei. | - |
dc.format | application/pdf | en |
dc.format.extent | 366855 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | en | en |
dc.language.iso | en_US | - |
dc.relation | Journal of Biomedical Science 15 (6): 833-840 | en |
dc.relation.ispartof | Journal of Biomedical Science | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | 2,3,7,8 tetrachlorodibenzo para dioxin; aromatic hydrocarbon receptor; cyan fluorescent protein; dioxin; hypoxia inducible factor 1beta; yellow fluorescent protein; agar gel electrophoresis; animal cell; article; bioassay; cancer cell culture; controlled study; fluorescence resonance energy transfer; hepatoma cell; nonhuman; plasmid; priority journal; rat; upregulation; Animals; Aryl Hydrocarbon Receptor Nuclear Translocator; Biological Assay; Cell Line, Tumor; Dioxins; Dose-Response Relationship, Drug; Fluoresceins; Fluorescence Resonance Energy Transfer; Rats; Receptors, Aryl Hydrocarbon; Teratogens; Tetrachlorodibenzodioxin; Rattus | - |
dc.title | Establishment of a fluorescence resonance energy transfer-based bioassay for detecting dioxin-like compounds | en |
dc.type | journal article | en |
dc.identifier.doi | 10.1007/s11373-008-9267-6 | - |
dc.identifier.scopus | 2-s2.0-56349142872 | - |
dc.identifier.isi | WOS:000260957100016 | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
dc.relation.pages | 833-840 | - |
dc.relation.journalvolume | 15 | - |
dc.relation.journalissue | 6 | - |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/161584/1/31.pdf | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
crisitem.author.dept | Life Science | - |
crisitem.author.dept | Neurobiology and Cognitive Science Center | - |
crisitem.author.orcid | 0000-0002-6654-9309 | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
顯示於: | 生命科學系 |
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