https://scholars.lib.ntu.edu.tw/handle/123456789/136522
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Tsai, Meng-Feng | en |
dc.contributor.author | Yu, Hon-Tsen | en |
dc.contributor.author | Tzeng, Huey-Fen | en |
dc.contributor.author | Leu, Jiann-Horng | en |
dc.contributor.author | Chou, Chih-Ming | en |
dc.contributor.author | Huang, Chang-Jen | en |
dc.contributor.author | Wang, Chung-Hsiung | en |
dc.contributor.author | Lin, Jung-Yaw | en |
dc.contributor.author | Kou, Guang-Hsiung | en |
dc.contributor.author | Lo, Chu-Fang | en |
dc.creator | Tsai, Meng-Feng; Yu, Hon-Tsen; Tzeng, Huey-Fen; Leu, Jiann-Horng; Chou, Chih-Ming; Huang, Chang-Jen; Wang, Chung-Hsiung; Lin, Jung-Yaw; Kou, Guang-Hsiung; Lo, Chu-Fang | - |
dc.date | 2000 | en |
dc.date.accessioned | 2009-06-24T06:13:35Z | - |
dc.date.accessioned | 2018-07-06T01:31:18Z | - |
dc.date.available | 2009-06-24T06:13:35Z | - |
dc.date.available | 2018-07-06T01:31:18Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 00426822 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034634328&doi=10.1006%2fviro.2000.0597&partnerID=40&md5=d051aac04182ebd511c19c5171935477 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/161585 | - |
dc.description.abstract | From previously constructed genomic libraries of a Taiwan WSSV isolate, a putative WSSV tk-tmk gene was identified. Uniquely, the open reading frame (ORF) of this gene was predicted to encode a novel chimeric protein of 388 amino acids with significant homology to two proteins: thymidine kinase (TK) and thymidylate kinase (TMK). Northern blot analysis with a WSSV tk-tmk-specific riboprobe detected a major transcript of 1.6 kb. When healthy adult Penaeus monodon shrimp were inoculated with WSSV, the tk-tmk gene transcript was first detected by RT-PCR analysis at 4 h postinfection and transcription levels continued to increase over the first 18 h. The gene's major in vitro transcription and translation product, equivalent to the predicted size (43 kDa), is a single chimeric protein that includes both the TK and TMK functional motifs. Evidence for phylogenetic analysis and sequence alignment suggested that the gene may have resulted from the fusion of a cellular-type TK gene and a cellular-type TMK gene. Its unique arrangement may also provide a valuable gene marker for WSSV. (C) 2000 Academic Press. | - |
dc.format | application/pdf | en |
dc.format.extent | 391278 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | en | en |
dc.language.iso | en_US | - |
dc.relation | Virology 277 (1): 100-110 | en |
dc.relation.ispartof | Virology | en_US |
dc.subject | Penaeus monodon; Taiwan WSSV isolate; Thymidine kinase; Thymidylate kinase; White spot syndrome virus; WSSV tk-tmk gene | - |
dc.subject.other | chimeric protein; polypeptide; thymidine kinase; article; DNA virus; genetic transcription; nonhuman; nucleotide sequence; open reading frame; phylogeny; priority journal; sequence analysis; sequence homology; shrimp; virus characterization; virus gene; White spot syndrome virus; Animalia; Decapoda (Crustacea); DNA viruses; Monodon; Penaeus monodon; Penaeus monodon; Shrimp white spot syndrome virus | - |
dc.title | Identification and Characterization of a Shrimp White Spot Syndrome Virus (WSSV) Gene That Encodes a Novel Chimeric Polypeptide of Cellular-Type Thymidine Kinase and Thymidylate Kinase | en |
dc.type | journal article | en |
dc.identifier.doi | 10.1006/viro.2000.0597 | - |
dc.identifier.pmid | 11062040 | - |
dc.identifier.scopus | 2-s2.0-0034634328 | - |
dc.relation.pages | 100-110 | - |
dc.relation.journalvolume | 277 | - |
dc.relation.journalissue | 1 | - |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/161585/1/07.pdf | - |
item.languageiso639-1 | en_US | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Life Science | - |
crisitem.author.orcid | 0000-0002-2911-0698 | - |
crisitem.author.parentorg | College of Life Science | - |
顯示於: | 生命科學系 |
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