https://scholars.lib.ntu.edu.tw/handle/123456789/137466
標題: | Vaccination of grouper broodfish (Epinephelus tukula) reduces the risk of vertical transmission by nervous necrosis virus | 作者: | Kai, Y.-H. Su, H.-M. Tai, K.-T. Chi, S.-C. |
關鍵字: | Fish nodavirus; Grouper; Inactivated vaccine; NNV; Vertical transmissions | 公開日期: | 2010 | 起(迄)頁: | 996-1001 | 來源出版物: | Vaccine | 摘要: | Nervous necrosis virus (NNV) has caused mass mortality in many species of cultured fish at larval stage. Strong evidence of vertical transmission of NNV has been reported in the carrier broodstock of striped jack and sea bass. An effective immunization program was developed and monitored in adult groupers (Epinephelus coioides) with average body weight of 1.35 kg. The highest neutralizing antibody titers were found in the fish intramuscularly injected with adjuvanted NNV vaccine at 109 TCID50 kg-1, and the enhanced 2-fold neutralization activity could sustain up to 17 months post-vaccination (mpv). An immunization program was applied in the broodstocks of grouper (Epinephelus tukula) with body weight of 35-60 kg. The levels of NNV-specific antibodies detected, from 1 to 5 mpi, in the homogenates of the eggs from the vaccinated broodfish were elevated than that from non-vaccinated fish. By nested RT-PCR, NNV was detectable in the eggs from the non-vaccinated fish at Month 5, but not in the eggs from vaccinated fish. It is therefore suggested that vaccination will be a potentially practical measure to reduce the risk of vertical transmission of NNV of grouper broodfish under stress of repeated spawning. ? 2009 Elsevier Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/205382 | DOI: | 10.1016/j.vaccine.2009.10.132 | SDG/關鍵字: | nervous necrosis virus vaccine; neutralizing antibody; unclassified drug; virus vaccine; animal experiment; antibody titer; article; body weight; controlled study; Epinephelus tukula; fish; immunization; nervous necrosis virus; nervous necrosis virus infection; nonhuman; priority journal; reverse transcription polymerase chain reaction; risk reduction; vaccination; vertical transmission; virus; virus infection; virus transmission; Animals; Antibodies, Neutralizing; Antibodies, Viral; Bass; Eggs; Fish Diseases; Immunologic Memory; Infectious Disease Transmission, Vertical; Injections, Intramuscular; Nodaviridae; Reverse Transcriptase Polymerase Chain Reaction; RNA Virus Infections; RNA, Viral; Time Factors; Vaccines, Inactivated; Viral Vaccines |
顯示於: | 生命科學系 |
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