|Title:||Hepatocellular carcinoma associated with focal nodular hyperplasia: report of a case with clonal analysis.||Authors:||Chen, TC
Wu Chou, Yah-Huei
|Issue Date:||Apr-2001||Journal Volume:||438||Journal Issue:||4||Start page/Pages:||408-411||Source:||Virchows Arch||Abstract:||
We describe a hepatocellular carcinoma partially surrounded by focal nodular hyperplasia in a 65-year-old female patient. In order to clarify the relationship of the hepatocellular carcinoma and the adjacent focal nodular hyperplasia, clonal analysis was conducted. The clonal analysis was based on the methylation pattern of the polymorphic X-chromosome-linked androgen receptor gene (HUMARA). The allelic bands from the amplification of the focal nodular hyperplasia and of the hepatocellular carcinoma showed a significant reduction in the intensity of one of the two alleles as compared with two alleles of equal intensity in the buff coat after HhaI digestion, which indicated that these two parts were monoclonal. However, the inactivated allele in the focal nodular hyperplasia and that in the hepatocellular carcinoma were not identical. Therefore, the focal nodular hyperplasia and hepatocellular carcinoma probably derived from the clonal expansion of two different clones.
|URI:||http://ntur.lib.ntu.edu.tw//handle/246246/192010||DOI:||10.1007/s004280000348||SDG/Keyword:||androgen receptor; restriction endonuclease; aged; allele; article; case report; disease association; DNA methylation; DNA polymorphism; female; gene amplification; human; human tissue; liver cell carcinoma; molecular cloning; nodular hyperplasia; priority journal; X chromosome linkage; Aged; Carcinoma, Hepatocellular; Clone Cells; DNA Primers; DNA, Neoplasm; Female; Focal Nodular Hyperplasia; Humans; Liver Neoplasms; Polymerase Chain Reaction; Receptors, Androgen
|Appears in Collections:||分子與細胞生物學研究所|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.