https://scholars.lib.ntu.edu.tw/handle/123456789/140572
標題: | Betel quid extract promotes oral cancer cell migration by activating a muscarinic M4 receptor-mediated signaling cascade involving SFKs and ERK1/2 | 作者: | Chiu, Chien-Chih Chen, Bing-Hung Hour, Tzyh-Chyuan Chiang, Wei-Fan Wu, Yu-Jen Chen, Ching-Yi Chen, Hau-Ren Chan, Po-Ting Liu, Shyun-Yeu Chen, Jeff Yi-Fu |
關鍵字: | Betel quid; ERK1/2; Muscarinic M4 receptor; Oral cancer cell migration; SFKs | 公開日期: | 2010 | 起(迄)頁: | 60-65 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | Betel quid (BQ) is a widely accepted etiological factor for oral squamous cell carcinoma (OSCC) in Southeast Asia, but how BQ chewing leads to oral carcinogenesis remains to be elucidated. We have previously demonstrated that the activation of Src family kinases (SFKs) is critical for BQ-induced oral cancer cell motility. Here we investigate whether this biological effect is mediated by specific membrane receptors in oral cancer cells. We found that BQ-induced activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and cell migration could be inhibited by atropine, suggesting the involvement of the muscarinic receptor family. The enhanced activities of ERK1/2 and cell migration were significantly counteracted by PD102807, the selective antagonist of muscarinic M4 receptor. Moreover, cold BQ extract effectively competed with a known ligand, [3H]-N-methyl scopolamine, for binding to muscarinic M4 receptor in vitro, thereby implying that BQ could activate motility-promoting signaling pathways through direct interaction with the receptor. The requirement of muscarinic M4 receptor for BQ-induced oral cancer cell migration was demonstrated by knockdown of the receptor using RNA interference (RNAi). Remarkably, ectopic expression of muscarinic M4 receptor in two oral cancer cell lines, Ca9-22 and SCC-9, further augmented BQ-induced cell migration by 83% and 99%, respectively. Finally, we verified that BQ-induced oral cancer cell migration was mediated through a muscarinic M4 receptor→SFKs→ERK1/2 signaling pathway. Thus, our findings have identified a novel signaling cascade mediating BQ-induced oral cancer cell motility, which could be a therapeutic target for BQ-related oral malignancies. ? 2010 Elsevier Inc. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/243301 | DOI: | 10.1016/j.bbrc.2010.07.042 | SDG/關鍵字: | betel extract; mitogen activated protein kinase 1; mitogen activated protein kinase 3; muscarinic M4 receptor; protein tyrosine kinase; article; cancer cell; cell migration; controlled study; enzyme activation; enzyme phosphorylation; genetic transfection; human; human cell; in vitro study; mouth cancer; priority journal; protein expression; RNA interference; signal transduction; Western blotting; Calcium Compounds; Cell Line, Tumor; Cell Movement; Humans; Mastication; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mouth Neoplasms; Oxides; Piper; Plant Extracts; Receptor, Muscarinic M4; Signal Transduction; Piper betel |
顯示於: | 分子與細胞生物學研究所 |
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