https://scholars.lib.ntu.edu.tw/handle/123456789/140687
Title: | 非光合作用被子植物質體基因研究 | Authors: | 胡哲明 | Keywords: | 非光合作用植物;質體基因組;non-photosynthetic plants;plastid genome;18S rDNA;16S rDNA;rbcL;matK | Issue Date: | 2004 | Publisher: | 臺北市:國立臺灣大學生態學與演化生物學研究所 | Abstract: | 葉綠體存在於所有綠色植物體內,為進行光合作用的場所。然而有些被子植物喪失光合作用的能力,成為全寄生或腐生的植物。這些非光合作用植物卻仍然保有一個縮減的質體基因組,其中大部分與光合作用相關的基因,如rbcL等,都已丟失或成為偽基因(pseudogene) 。本計畫即以九種台灣的非綠色被子植物為材料,進行其質體基因的分析,探討質體基因的演化。結果共獲得九條18S rDNA、七條16SrDNA序列,並依據其序列進行譜系分析。另外穗花蛇菰、無根藤和列當均利用PCR得到接近全長的rbcL與matK序列。但是在菟絲子中只得到rbcL,而野菰只能得到matK的序列。而RT-PCR的結果顯示菟絲子的rbcL有微量表現,應具有些微光合作用能力。 Chloroplasts are the eukaryotic organelles mainly responsible for photosynthesis. There are some flowering plants, however, lost the photosynthetic ability and became holoparasitic or saprophytic plants. The non-photosynthetic plants usually retain a reduced plastid genome, although many of plastid genes are lost or become pseudogenes, especially the photosynthesis-related genes such as rbcL. In order to elucidate the plastid genome evolution in non-photosynthetic plants, we examined nine non-photosynthetic plants native to Taiwan. We obtained all 18S rDNA sequences and seven 16S rDNA sequences of the examined taxa, and further using the sequences to perform phylogenetic analyses. In Balanophora, Cassytha and Orobanche, we obtained almost full length rbcL and matK sequences. However, we only obtained rbcL from Cuscuta and matK sequence from Aeginetia. The RT-PCR result shows that the rbcL transcripts were detected in Cuscuta, and suggests Cuscuta may have retain photosynthetic ability. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/10816 | Other Identifiers: | 922311B002026 | Rights: | 國立臺灣大學生態學與演化生物學研究所 |
Appears in Collections: | 生態學與演化生物學研究所 |
File | Description | Size | Format | |
---|---|---|---|---|
922311B002026.pdf | 1.05 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.