https://scholars.lib.ntu.edu.tw/handle/123456789/142197
Title: | PORTABLE MICROSATELLITE PRIMERS FOR FICUS (MORACEAE) | Authors: | Garcia, Marjorie Bain, Anthony Tzeng, Hsy-Yu Peng, Yan-Qiong Chou, Lien-Siang Kjellberg, Finn |
Keywords: | Coevolution; Ficus; Moraceae; Mutualism | Issue Date: | 2012 | Start page/Pages: | e187-e192 | Source: | American Journal of Botany | Abstract: | Premise of the study: Highly portable microsatellite primers were developed for Ficus to facilitate investigation of genetic structure of complete regional floras using a single set of markers. Methods and Results: Pyrosequencing of five species of Ficus produced a library of 5723 potential primers. Potential primers found in at least two species and presenting identical annealing temperatures were tested on a set of five additional Ficus species. A set of 20 primer pairs producing well-defined and easily readable peaks was retained and tests showed their potential utility for analyzing population genetic structure of 24 Ficus species from Taiwan. Numbers of alleles per locus ranged from one to six in the least variable species and from one to 17 in the most variable species. Conclusions: The results indicate that our set of primers can be used to analyze polymorphism and compare levels of polymorphism among Ficus species. © 2012 Botanical Society of America. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84860589049&doi=10.3732%2fajb.1100485&partnerID=40&md5=1b142ba28eb120e43076439d3bd6e60d http://ntur.lib.ntu.edu.tw//handle/246246/243368 |
ISSN: | 00029122 | DOI: | 10.3732/ajb.1100485 | SDG/Keyword: | microsatellite DNA; primer DNA; allele; coevolution; cohort analysis; dicotyledon; genetic marker; genetic structure; mutualism; polymorphism; article; Ficus; gene locus; genetic variability; genetics; metabolism; molecular genetics; nucleotide sequence; DNA Primers; Ficus; Genetic Loci; Genetic Variation; Microsatellite Repeats; Molecular Sequence Data; Ficus (angiosperm); Moraceae |
Appears in Collections: | 生態學與演化生物學研究所 |
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