https://scholars.lib.ntu.edu.tw/handle/123456789/142422
標題: | 海洋微細藻類之抗癌、抗病毒藥物開發與其他活性物質探討 | 作者: | 周宏農 | 關鍵字: | 微細藻;抗癌活性;活性物質;大環內酯;microalgae, anti-cancer, bioactive compound, macrolide | 公開日期: | 31-七月-2003 | 出版社: | 臺北市:國立臺灣大學漁業科學研究所 | 摘要: | 本研究的目的,在於由微細藻中找尋具有抗癌、抗病毒活性的物質,作為後續藥物開 發的基礎。先期由微細藻種源庫的建立著手,於計畫期間共計由本省各地的採樣中純化出 206 株藻,提供理想的材料進行後續的研究。在初步的活性分析中,發現其中至少有39 株 具有明顯的細胞毒殺活性,以渦鞭毛藻的端溝藻Amphinidium 及原甲藻Prorocentrum 發現 活性的比例最高。後續由其中的4 個藻株純化出26 個化合物;在其中已完成結構鑑定的 19 個化合物中,發現1 個new skeleton 及5 個新化合物;其餘7 個結構尚未完全解開的化 合物預期均為新化合物,甚至new skeleton 化合物。所有化合物中,至少已知有6 種以上 具有明顯的細胞毒殺活性,顯示省產微細藻類於新型活性化合物開發的潛力與價值。 過去已知存在於海洋生物中的大環內酯化合物macrolides 均有極強的細胞毒殺活性, 然因結構複雜,產量低,因此難以開發成為抗癌藥物,研究中發現的prorocentrolides 化合 物亦不例外。然藉由Prorocentrum 藻株的篩檢,成功挑取出該類化合物含量超高的藻株, 足以克服產量及後續生合成實驗的困難,如此更凸顯出微細藻種源庫的價值。 This project aimed on the exploitation of bioactive compounds from microalgae to match the need of NHRI in drug development. For the purpose, algae indigenous to Taiwan were isolated, cultivated, and harvested for the previous screening of cytotoxicities. It was found dinoflagellate belonging to the genus of Amphidinium and Prorocentrum exerted strong cytotoxicities with high frequency. The potentially active strains of these genuses were cultivated to larger volume for purifying enough bioactive compounds inside. By the way, twenty-six compounds were purified from four Amphidinium and Prorocentrum strains respectively, and the structures of 19 compounds were fully elucidated. Five of them were new compounds and 1 with new skeleton; other 7 compounds whose structures were partially elucidated were identified as new compounds or new skeletal compounds. At least 6 of them exerted strong cytotoxicities, indicating the potency to explore anti-cancer drugs from the microalgae of Taiwan. Macrolides produced by marine creatures were known to exerted strong cytotoxicity. The complex structures and the low contents, however, restricted their utilizations on cancer therapy, with no exceptions for prorocentrolides found in our research. It was overcome, however, by finding a strain of Prorocentrum with high prorocentrolide content, indicating the value to keep a library of algal strains. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/20560 | 其他識別: | 912323B002009 | Rights: | 國立臺灣大學漁業科學研究所 |
顯示於: | 漁業科學研究所 |
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912323B002009.pdf | 108.06 kB | Adobe PDF | 檢視/開啟 |
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