https://scholars.lib.ntu.edu.tw/handle/123456789/143414
Title: | The zebrafish erythropoietin: Functional identification and biochemical characterization | Authors: | Chu, Cheng-Ying Cheng, Chia-Hsiung Chen, Gen-Der Chen, Yi-Chung Hung, Chin-Chun Huang, Kai-Yun Huang, Chang-Jen |
Keywords: | Erythropoietin; Globin staining; Glycoprotein; Morpholino oligonucleotide; Secretion; Zebrafish | Issue Date: | 2007 | Start page/Pages: | 4265-4271 | Source: | FEBS Letters | Abstract: | In the present study, the zebrafish epo cDNA was cloned. The encoded protein displays 90%, 55% and 32% identity to the Epo from carp, fugu and human, respectively. Through RT-PCR, the expression of zepo mRNA was mainly in the heart and liver. In the COS-1 cell transfection experiments, the recombinant zEpo-HA protein was efficiently secreted into the culture medium as a glycoprotein and the carbohydrate moiety can be cleaved by the treatment of peptide-N-glycosidase F (PNGase F). Using the morpholino approach, we showed that zepo morphants displayed severe anemia leading to high mortality during development. Such an effect can be significantly rescued by zepo RNA. Furthermore, in the absence of functional zEpo, the expression of specific markers for adult globin genes, such as αA1- and βA1-globin, but not the embryonic βe1-globin, was affected. ? 2007 Federation of European Biochemical Societies. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/162991 | DOI: | 10.1016/j.febslet.2007.07.073 | SDG/Keyword: | complementary DNA; erythropoietin; glycopeptidase; hemoglobin alpha chain; hemoglobin beta chain; messenger RNA; anemia; animal cell; animal tissue; article; cell strain COS1; controlled study; culture medium; gene expression; genetic transfection; molecular cloning; mortality; nonhuman; nucleotide sequence; priority journal; protein analysis; protein degradation; protein function; protein secretion; real time polymerase chain reaction; zebra fish; Amino Acid Sequence; Animals; Cercopithecus aethiops; Cloning, Molecular; COS Cells; Embryo, Nonmammalian; Erythroid Cells; Erythropoietin; Gene Expression Profiling; Gene Expression Regulation, Developmental; Hemoglobins; Molecular Sequence Data; Organ Specificity; Protein Transport; RNA, Messenger; Sequence Alignment; Zebrafish; Zebrafish Proteins; Cyprinidae; Danio rerio; Takifugu |
Appears in Collections: | 生化科學研究所 |
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