https://scholars.lib.ntu.edu.tw/handle/123456789/144026
標題: | A Cholesterol Biosynthesis Inhibitor Blocks Staphylococcus aureus Virulence | 作者: | Liu, Chia-I Liu, George Y. Song, Yongcheng Yin, Fenglin Hensler, Mary E. Jeng, Wen-Yih Nizet, Victor Wang, Andrew H.-J. Oldfield, Eric |
公開日期: | 2008 | 起(迄)頁: | 1391-1394 | 來源出版物: | Science | 摘要: | Staphylococcus aureus produces hospital- and community-acquired infections, with methicillin-resistant S. aureus posing a serious public health threat. The golden carotenoid pigment of S. aureus, staphyloxanthin, promotes resistance to reactive oxygen species and host neutrophil-based killing, and early enzymatic steps in staphyloxanthin production resemble those for cholesterol biosynthesis. We determined the crystal structures of S. aureus dehydrosqualene synthase (CrtM) at 1.58 angstrom resolution, finding structural similarity to human squalene synthase (SQS). We screened nine SQS inhibitors and determined the structures of three, bound to CrtM. One, previously tested for cholesterol-lowering activity in humans, blocked staphyloxanthin biosynthesis in vitro (median inhibitory concentration ?100 nM), resulting in colorless bacteria with increased susceptibility to killing by human blood and to innate immune clearance in a mouse infection model. This finding represents proof of principle for a virulence factor-based therapy against S. aureus. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/163379 | DOI: | 10.1126/science.1153018 | SDG/關鍵字: | bacterial enzyme; bph 651; bph 652; bph 660; bph 661; bph 673; bph 674; bph 698; bph 700; carotenoid; dehydrosqualene synthase; farnesyl thiodiphosphate; squalene synthase; squalene synthase inhibitor; staphyloxanthin; unclassified drug; bacterium; biochemistry; carotenoid; crystal structure; disease treatment; enzyme activity; immune system; inhibitor; metabolism; rodent; sterol; virulence; animal experiment; animal model; article; bacterial virulence; bactericidal activity; blood; cholesterol synthesis; controlled study; crystal structure; drug screening; drug structure; enzyme structure; in vitro study; inhibition kinetics; innate immunity; mouse; nonhuman; priority journal; Staphylococcus aureus; Staphylococcus infection; structure analysis; Amino Acid Sequence; Animals; Anti-Bacterial Agents; Bacterial Proteins; Cell Line; Cell Proliferation; Cholesterol; Crystallography, X-Ray; Enzyme Inhibitors; Farnesyl-Diphosphate Farnesyltransferase; Humans; Mice; Molecular Sequence Data; Organothiophosphorus Compounds; Polyisoprenyl Phosphates; Protein Structure, Secondary; Sesquiterpenes; Staphylococcal Infections; Staphylococcus aureus; Virulence; Xanthophylls; Staphylococcus aureus |
顯示於: | 生化科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。