|Title:||Tumour Necrosis Factor-Alpha-Induced Apoptosis in Cord Blood T Lymphocytes: Involvement of Both Tumour Necrosis Factor Receptor Types 1 and 2||Authors:||YANG, YA-CHIEN
|Keywords:||cord blood;T cells, apoptosis;TNFR1;TNFR2;VERSUS-HOST DISEASE;UNRELATED DONORS||Issue Date:||2001||Journal Volume:||v.115||Journal Issue:||n.2||Start page/Pages:||435-441||Source:||BRITISH JOURNAL OF HAEMATOLOGY||Abstract:||
Cord blood T cells are much more likely to be induced to apoptosis in vitro than adult T cells. Nevertheless, the expression of Fas is markedly lower on cord blood lymphocytes than on peripheral blood lymphocytes. In the current investigation, we determined the capacity of tumour necrosis factor-alpha (TNF-alpha) to induce apoptosis in human naive T cells in cord blood, and assessed the roles of two distinct TNF receptors (TNFRs) in mediating death signals. After activation, cord blood T cells were sensitive to TNF-alpha -induced apoptosis, and interleukin 2 (IL-2) could prevent this apoptotic response. Both TNFR1 (p55) and TNFR2 (p75) expressed on activated cord blood T cells were able to transmit apoptotic signals. Moreover, a synergistic effect was observed by a combination of TNFR1- and TNTFR2- signals. Additionally, CD4(+) T cells showed higher sensitivity to TNTR-mediated apoptosis than CD8(+) T cells. These data suggest that TNF-alpha probably is a mediator of apoptosis in cord blood T cells in vivo and may contribute to the low incidence of graft-versus- host disease in cord blood transplantation.
|Appears in Collections:||醫學檢驗暨生物技術學系|
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