https://scholars.lib.ntu.edu.tw/handle/123456789/146115
標題: | The Luxr Family Protein Spnr Functions as a Negative Regulator of N- Acylhomoserine Lactone-Dependent Quorum Sensing in Serratia Marcescens | 作者: | HO, SHEN-WU LAI, HSIN-CHIH |
關鍵字: | SWARM-CELL-DIFFERENTIATION;EXTRACELLULAR NUCLEASE;LIQUEFACIENS MG1;N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE;ANTIBIOTIC BIOSYNTHESIS;MULTICELLULAR BEHAVIOR | 公開日期: | 2002 | 卷: | v.45 | 期: | n.6 | 起(迄)頁: | 1655-1671 | 來源出版物: | MOLECULAR MICROBIOLOGY | 摘要: | Serratia marcescens SS-1 produces at least four N - acylhomoserine lactones (AHLs) which were identified using high-resolution mass spectrometry and chemical synthesis, as N-(3-oxohexanoyl) homo-serine lactone (3-oxo-C6-HSL), N- hexanoyl- (C6-HSL), N-heptanoyl (C7-HSL) and N- octanoyl- (C8 -HSL) homoserine lactone. These AHLs are synthesized via the LuxI homologue SpnI, and regulate via the LuxR homologue SpnR, the production of the red pigment, prodigiosin, the nuclease, NucA, and a biosurfactant which facilitates surface translocation. spnR overexpression and spnR gene deletion show that SpnR, in contrast to most LuxR homologues , acts as a negative regulator. spnI overexpression, the provision of exogenous AHLs and spnI gene deletion suggest that SpnR is de -repressed by 3-oxo-C6-HSL. In addition, long chain AHLs antagonize the biosurfactant-mediated surface translocation of S. marcescens SS-1. Upstream of spnI there is a gene which we have termed spnT. spnI and spnT form an operon and although database searches failed to reveal any spnT homologues, overexpression of this novel gene negatively affected both sliding motility and prodigiosin production. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/105270 |
顯示於: | 醫學檢驗暨生物技術學系 |
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