https://scholars.lib.ntu.edu.tw/handle/123456789/146419
標題: | Human Tlr3 Recognizes Dengue Virus and Modulates Viral Replication in Vitro | 作者: | TSAI, YI-TING CHANG, SUI-YUAN LEE, CHUN-NAN KAO, CHUAN-LIANG |
公開日期: | 2009 | 卷: | v.11 | 期: | n.4 | 起(迄)頁: | 604-615 | 來源出版物: | CELLULAR MICROBIOLOGY | 摘要: | The elicitation of large amount inflammatory cytokine in serum has been developed as the cause of the plasma leakage in dengue fever (DF)/dengue haemorrhagic fever (DHF) infection. Virus recognition in innate immunity is the key. The Toll-like receptors (TLRs) play an important role in pathogen recognition towards cytokine induction among several viruses; however, the role of TLRs on innate immune recognition against DENV remains unclear. This study aims at the interaction between dengue virus ( DENV) and human TLRs at the incipient stage of infection in vitro. Our experiment reveals that stably expression of TLR3, 7, 8 on HEK293 enables IL-8 secretion after DENV recognition. By the model of human monocytic cells U937, we demonstrated the trigger of IL-8 after viral recognition of human monocytic cell is primary through TLR3 following endosomal acidification. Silencing of TLR3 in U937 cells significantly blocks the DENV-induced IL-8 production. Besides, the interaction is further corroborated by colocalization of TLR3 and DENV RNA upon DENV internalization. Furthermore, in this study we found the expression of TLR 3 can mediate strong IFN-alpha/ beta release and inhibit DENV viral replication significantly, thus limit the cytopathic effect. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/188813 |
顯示於: | 醫學檢驗暨生物技術學系 |
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