https://scholars.lib.ntu.edu.tw/handle/123456789/160028
標題: | The Yeast Rna-Binding Protein Rbp1p Modifies the Stability of Mitochondrial Porin Mrna | 作者: | BUU, LEH-MIAUH LEE, FANG-JEN S |
公開日期: | 2004 | 卷: | v.279 | 期: | n.1 | 起(迄)頁: | 453-462 | 來源出版物: | JOURNAL OF BIOLOGICAL CHEMISTRY | 摘要: | The Saccharomyces cerevisiae RNA-binding protein Rbp1p was initially identified as a negative growth regulator; however , its function is still obscure. Here, we show that Rbp1p in cells is associated with structures that sediment at 10,000 as well as 100,000 x g. It appears microscopically as punctate signals partially localized to the perinuclear region. Over- expression of Rbp1p in yeast resulted in growth defects on nonfermentable carbon sources, suggesting a function for Rbp1p in mitochondrial biogenesis. Absence of Rbp1p increased the level of mitochondrial porin, whereas over-expression of Rbp1p, but not an N-terminally truncated form, decreased porin levels. Over-expression of Rbp1p also decreased the level of mitochondrial porin mRNA by enhancing its degradation, an effect that was dependent on all three of the Rbp1p RNA recognition motifs. In cells, the porin mRNA is associated with Rbp1p . RNP ( ribonucleoprotein) complexes. In vitro binding assays showed that Rbp1p most likely interacts with a (C/G) U-rich element in the porin mRNA 3'-UTR. Based on these observations, we infer that Rbp1 p has a role in negatively regulating mitochondrial porin expression post-transcriptionally. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/92454 |
顯示於: | 分子醫學研究所 |
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