https://scholars.lib.ntu.edu.tw/handle/123456789/160035
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 分子醫學研究所 | en |
dc.contributor.author | BUU, LEH-MIAUH | en |
dc.contributor.author | LEE, FANG-JEN S | en |
dc.creator | 卜樂妙;李芳仁 | zh-tw |
dc.creator | BUU, LEH-MIAUH;LEE, FANG-JEN S | en |
dc.date | 2006 | en |
dc.date.accessioned | 2008-12-22T02:56:28Z | - |
dc.date.accessioned | 2018-07-09T01:15:02Z | - |
dc.date.available | 2008-12-22T02:56:28Z | - |
dc.date.available | 2018-07-09T01:15:02Z | - |
dc.date.issued | 2006 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/92461 | - |
dc.description.abstract | Rbp1p, a yeast RNA-binding protein, decreases the level of mitochondrial porin mRNA by enhancing its degradation, but the intracellular location of the Rbp1p-mediated degradation complex remains unknown. We show here that Rbp1p in xrn1 Delta mutant yeast localizes in specific cytoplasmic foci that are known as P-bodies. The N-terminal and RNA recognition motif (RRM) 1 domains of Rbp1p are necessary but not sufficient for its localization in P bodies. Rbp1p forms oligomers through its C-terminal domain in vivo; N- terminal-delete, or RRM1-mutated Rbp1p can be more efficiently recruited to P-bodies in an xrn1 Delta strain, expressing a full-length Rbp1p. Although POR1 mRNA is localized to P bodies in an xrn1 Delta strain , this localization does not depend on Rbp1p. Decapping activator Dhh1p directly interacts with Rbp1p. However, the recruitment of Rbp1p to P- bodies does not require Dhh1p or Ccr4p. In wildtype cells, Rbp1p can localize to P-bodies under glucose deprivation or treatment with KCl. In addition , Rbp1p-mediated porin mRNA decay is elicited by Xrn1p, a 5' to 3 ' exonuclease. These results provide new insight into the mechanism of Rbp 1p function. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | JOURNAL OF BIOLOGICAL CHEMISTRY v.281 n.39 pp.29379-29390 | en |
dc.relation.ispartof | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.title | Determinants of Rbp1p Localization in Specific Cytoplasmic Mrna- Processing Foci, P-Bodies | en |
dc.relation.pages | 29379-29390 | - |
dc.relation.journalvolume | v.281 | - |
dc.relation.journalissue | n.39 | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
顯示於: | 分子醫學研究所 |
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