https://scholars.lib.ntu.edu.tw/handle/123456789/161830
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 動物中心 | en |
dc.contributor.author | SHYU, RONG-YAUN | en |
dc.contributor.author | JIANG, SHUN-YUAN | en |
dc.contributor.author | JONG, YIIN-JENG | en |
dc.contributor.author | CHENG, KUO-CHEN | en |
dc.contributor.author | LIN, CHI-HUNG | en |
dc.contributor.author | YU, JYH-CHERNG | en |
dc.contributor.author | WU, MING-FANG | en |
dc.contributor.author | CHANG, TZU-MING | en |
dc.creator | 徐榮源;姜淑媛;鍾尹禎;鄭高珍;林奇宏;俞志誠;吳銘芳;張子明 | zh_TW |
dc.creator | SHYU, RONG-YAUN;JIANG, SHUN-YUAN;JONG, YIIN-JENG;CHENG, KUO-CHEN;LIN, CHI-HUNG;YU, JYH-CHERNG;WU, MING-FANG;CHANG, TZU-MING | en |
dc.date | 2004 | en |
dc.date.accessioned | 2009-01-19T02:41:43Z | - |
dc.date.accessioned | 2018-07-09T04:27:15Z | - |
dc.date.available | 2009-01-19T02:41:43Z | - |
dc.date.available | 2018-07-09T04:27:15Z | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/99423 | - |
dc.description.abstract | Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61 %, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and beta-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53 . The cell had the deletion at chromosome 18q and gains at chromosome 2p13 -25, 5p15, 5q21- 35, 7, 8q24, 9q, 11, 12p, 14q24-32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7 qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27-32% by interferon-alpha (2,000 U/ml) and by interferon-gamma with an IC50 of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-alpha and interferon-gamma. It can be used to develop new modalities of human gastric cancer treatment. Copyright (C) 2004 S. Karger AG, Basel. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | CELLS TISSUES ORGANS v.177 n.1 pp.37-46 | en |
dc.relation.ispartof | CELLS TISSUES ORGANS | - |
dc.subject | gastric cancer | en |
dc.subject | cell line | en |
dc.subject | interferon | en |
dc.subject | karyotypic aberration | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Establishment and Characterization of a Human Gastric Carcinoma Cell Line Tmc-1 | en |
dc.relation.pages | 37-46 | - |
dc.relation.journalvolume | v.177 | - |
dc.relation.journalissue | n.1 | - |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
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