|Title:||An Acute Injection of Porphyromonas Gingivalis Lipopolysaccharide Modulates the Opg/Rankl System and Interleukin-6 in an Ovariectomized Mouse Model||Authors:||Lu, H.-K.
|Keywords:||interleukin-6;osteoporosis;osteoprotegerin;Porphyromonas gingivalis;lipopolysaccharide;receptor activator of nuclear factor-kappa B ligand||Issue Date:||2008||Source:||Oral Microbiology and Immunology||Start page/Pages:||220-225||Abstract:||
Background/aims: In the present study, we attempted to develop a simulated model to explore the causal effects of periodontal pathogens on skeletal homeostasis in postmenopausal osteoporosis. Methods: Fifty-three female adult ICR mice were randomly assigned to an experimental group ( ovariectomized) or a control group. A single injection of Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS, ATCC 33277) or Escherichia coli lipopolysaccharide (E. coli-LPS) was administered intraperitoneally 4 weeks after an ovariectomy. Concentrations of interleukin-6 (IL-6), osteoprotegerin (OPG ), and the receptor activator of nuclear factor-kappa B ligand (RANKL) in serum were subsequently analyzed using an enzyme-linked immunosorbent assay (ELISA). Results: Under stimulation with P. gingivalis-LPS or E. coli-LPS, the concentration of OPG rose in both groups. The serum level of RANKL showed a decreasing trend 24 h after the injection in both groups. After injection of P. gingivalis-LPS in both the experimental and control animals, the OPG : RANKL ratio increased 24 h after the booster (22.26-620.99, P < 0.05). The serum level of IL-6 in the experimental group significantly increased 1-6 h after administration of E. coli-LPS and 1-3 h after administration of P . gingivalis-LPS (P < 0.05). Conclusions: A single booster injection of P. gingivalis-LPS induced short-term changes in OPG, RANKL, and IL-6 serum levels in this ovariectomized mouse model.
|Appears in Collections:||實驗動物中心|
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