DC 欄位 | 值 | 語言 |
dc.contributor | 郭彥彬 | zh-TW |
dc.contributor | Kuo, Mark Yen-Ping | en |
dc.contributor | 臺灣大學:臨床牙醫學研究所 | zh-TW |
dc.contributor.author | 林佼穎 | zh-TW |
dc.contributor.author | Lin, Chiao-Ying | en |
dc.creator | 林佼穎 | zh-TW |
dc.creator | Lin, Chiao-Ying | en |
dc.date | 2008 | en |
dc.date.accessioned | 2010-05-26T05:07:53Z | - |
dc.date.accessioned | 2018-07-09T04:53:29Z | - |
dc.date.available | 2010-05-26T05:07:53Z | - |
dc.date.available | 2018-07-09T04:53:29Z | - |
dc.date.issued | 2008 | - |
dc.identifier.other | U0001-2907200812080600 | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/184164 | - |
dc.description.abstract | 在正常口腔黏膜,ANX A1主要表現在細胞膜,但在分化良好的細胞核亦見其表現;在上皮變異(OED)的病例,則有位置移轉的現象:自細胞膜移向細胞質,或細胞核;而口腔鱗狀上皮細胞癌(OSCC)的病例則,可見ANX A1在細胞核的表現,有增加的現象,其中在OED核的正表現比率為13.64%,即66個病例有9個有正表現;而OSCC則在115個病例中有63個細胞核正表現的現象(54.8%)。其中以Kaplan-Meier曲線分析,顯示OSCC的細胞核陽性表現與較短的存活率,在統計上有意義的相關(P:0.00036)。多變數分析(stepwise multivariate logistic-regression model),則顯示出ANX A1核染色可用來做一為較差存活的指標。另外,在口腔鱗狀上皮細胞癌細胞株SAS,投予肝生長因子(HGF)可誘發ANX A1蛋白質自細胞膜質移向細胞核。而以PI3K的抑制劑LY294002幾乎可以完全抑制(88.3%)由HGF所引起的ANX A1之細胞核移轉。 | zh-TW |
dc.description.abstract | To investigate whether annexin A1 (ANXA1) expression is a marker in predicting the prognosis of oral cancer patients. We immunohistochemically examined the expression of ANXA1 in 66 cases of oral epithelial dysplasia (OED) and 115 cases of oral squamous cell carcinoma (OSCC). The results were correlated with the clinicopathological parameters of tumors and overall patient survival. In normal oral mucosa, ANXA1 staining was predominantly located on the cell membrane. In OED and OSCC specimens, membranous staining decreased, whereas nuclear staining increased. Positive nuclear staining was observed in 9 of 66 (13.64%) OED cases and 63 of 115 (54.8%) OSCCs. Kaplan–Meier curves showed that OSCC patients with ANXA1 nuclear staining had significantly shorter overall lengths of survival (P¼0.00036 by the log-rank test). Multivariate analysis showed that ANXA1 nuclear staining is a significant predictor of poor overall survival. And oral cancer SAS cells treated with hepatocyte growth factor (HGF) can induce ANXA1 protein translocation from cytoplasm to nucleus. Cells pretreated with LY294002 (PI3K inhibitor) almost completely inhibited (88.3% inhibition) HGF-mediated ANXA1 nuclear translocation. The nuclear localization of ANXA1 protein is a frequent event and could be used as a prognostic factor in OSCC. | en |
dc.description.tableofcontents | 口試委員會審定書…………………………………………………………………I謝…………………………………………………………………………………II文摘要……………………………………………………………………………III文摘要……………………………………………………………………………IV一章 序論.1 第一節 口腔鱗狀細胞癌(OSCC) ……………………………………… 1.2 第二節 Annexin 家族導論Introduction: Overview of the Annexin Family)………… 2.2.1 基因組成與演化歷史Gene organization & revolutionary history) …………… 2.2.2 結構特色(Characteristic structural features) …………… 3.2.3 位置與多元化功能(Localization & Functional Diversity)… 5.2.3.1 Annexin 通論………………………………………………… 5.2.3.2 ANX A1 個論…………………………………………………… 7.3 第三節 Annexin 與疾病的關係 (Annexin and Diseases) ……… 11.3.1 心臟與循環的疾病 (Disorders of the Heart and Circulation) …… 11.3.1.1 Annexin及心血管切片(Annexins and cardiova scular biology)11.3.1.2 心臟疾病 (Heart diseases) ……………………………… 12.3.1.3 Annexin 與抗凝血 (Annexins as anticoagulants) …………… 13.3.2 ANX與生理學壓力(Annexins and Physiological Stress) ……………14.3.3 ANX 與其他疾病…………………………………………………… 15.3.4. ANX 與癌症(Annexins and Cancer) …………………………………15.3.4.1 ANX 與癌症………………………………………………………16.3.4.2 ANX A1 與癌症(見表1) ………………………………………17二章 材料與方法……………………………………………………………… 23.1 第一節 檢體之取樣與收集…………………………………………… 23.1.1 臨床資料…………………………………………………………… 23.1.2 標本之採集………………………………………………………… 23.1.3 標本之處理………………………………………………………… 24.2 第二節 實驗方法……………………………………………………… 24.2.1 蘇木紫與伊紅染色………………………………………………… 24.2.2 免疫組織化學染色………………………………………………… 24.2.2.1 原理免疫組織化學染色……………………………………… 24.2.2.2 實驗步驟……………………………………………………… 26.2.3 組織病理檢查及組織學分類……………………………………… 28.2.4 組織之核糖核酸萃取 (Total RNA extraction from tissues) 28.2.5 反轉錄-聚合酶鏈鎖反應(RT-PCR)……………………………… 29.2.6 細胞培養(Cell culture) ………………………………………… 31.2.7 抑制劑的處理 (inhibitor treatment) ………………………… 32.2.8 免疫螢光染色 (Immunofluorescence Stains) ………………… 33.2.9 西方墨點法 (Western blot) …………………………………… 33.3 第三節 試劑 (Reagent) …………………………………………… 35.4 第四節 溶液備製……………………………………………………… 35.5 第五節 統計方法……………………………………………………… 36三章 結果……………………………………………………………………… 37.1 第一節 ANX A1 在口腔正常上皮(normal oral epithelial, NOE)口腔皮變異(oral epithelial dysplasia ,OED)及口腔鱗狀上皮癌(OSCC)的表…………………………………………………………………………………… 37.2 第二節 免疫化學組織染色結果與臨床病理因子的關係…………… 37.3 第三節 以反轉錄-聚合酶鏈鎖反應(RT-PCR)的方式,探討ANX A1 在腔鱗狀上皮細胞癌組織的表現 ………………………………………38.4 第四節 肝生長激素刺激ANX A1 且細胞質移位至細胞核於口腔鱗狀上癌細胞株SAS (Stimulation of ANXA1 Nuclear Translocation in Humanral Cancer SAS Cells byGF) ………………………………………………………………………38.5 第五節 由HGF 引起的ANX A1 核移位經由PI3K 訊息傳導路徑 …… 39.6 第六節 ANX A1 在口腔鱗狀上皮細胞癌細胞株的表現(Annexin AI mRNAxpression in the oral SCC cell lines) ………………………… 40.6.1 mRNA 的表現………………………………………………………… 40.6.2 蛋白質的表現……………………………………………………… 40.6.3 ANX A1 在台灣口腔鱗狀上皮細胞癌細胞株TW2.6 的表現……… 41.7 第七節 補遺資料(Supplement Data) ……………………………… 41.7.1 c-met 免疫組織化學染色………………………………………… 41四章 討論……………………………………………………………………… 42amp;#63851;考文獻…………………………………………………………………………… 47錄-圖………………………………………………………………………………58………………………………………………………………………………64表文章(Publications) …………………………………………………………69 | en |
dc.format | application/pdf | en |
dc.format.extent | 2993269 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | en |
dc.language.iso | en_US | - |
dc.subject | ANX A1 | zh-TW |
dc.subject | 口腔鱗狀上皮細胞癌 | zh-TW |
dc.subject | 細胞核移轉 | zh-TW |
dc.subject | annexin A1 | en |
dc.subject | oral squamous cell carcinoma | en |
dc.subject | nuclear translocation | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Annexin A1基因表現在口腔鱗狀細胞癌之研究 | zh-TW |
dc.title | Annexin A1 expression in oral squamous cell carcinoma | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/184164/1/ntu-97-D88422001-1.pdf | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | open | - |
item.fulltext | with fulltext | - |
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