DC 欄位 | 值 | 語言 |
dc.contributor | 郭彥彬 | zh-TW |
dc.contributor | 臺灣大學:口腔生物科學研究所 | zh-TW |
dc.contributor.author | 吳芳儀 | zh-TW |
dc.contributor.author | Wu, Fang-Yi | en |
dc.creator | 吳芳儀 | zh-TW |
dc.creator | Wu, Fang-Yi | en |
dc.date | 2009 | en |
dc.date.accessioned | 2010-05-04T06:18:23Z | - |
dc.date.accessioned | 2018-07-09T05:26:24Z | - |
dc.date.available | 2010-05-04T06:18:23Z | - |
dc.date.available | 2018-07-09T05:26:24Z | - |
dc.date.issued | 2009 | - |
dc.identifier.other | U0001-1407200916130900 | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/178615 | - |
dc.description.abstract | 口腔癌為台灣男性十大癌症發生率的第五位。也是台灣地區年增率最高的癌症。儘管近幾年口腔癌在診斷及治技術上有改進,但病人五年存活率並未有顯著改善。因此須尋找新的治療與預防方法來改善整理存活率及生活品質。Lovastatin為膽固醇合成路徑HMG-CoA reductase的抑制劑,臨床上用以降低病人高血脂。近年研究指出,lovastatin可造成大腸癌、乳癌、及頭頸癌細胞凋亡,但其機制並不十分清楚。因此本研究以人類口腔癌細胞株Ca9-22來探討它對口腔癌細胞的影響。結果顯示,以lovastatin處理Ca9-22細胞可明顯抑制其細胞生長,且濃度越高效應更加明顯。西方墨點分析顯示,以4uM的lovastatin處理Ca9-22,subG0/G1細胞數目百分比會明顯的增加,表示 lovastatin可以引起人類口腔癌細胞凋亡。西方墨點法實驗發現,在內生性凋亡路徑中,lovastatin會增加cyochrome c的釋放,以活化下游 caspase-9造成細胞凋亡。但外生性路徑之細胞膜上死亡受體 (TRAILR1、TRAILR2及FAS)皆沒有明顯改變。加入caspase-8和-9抑制劑 (Z-LEHD-FMK, Z-IETD-FMK)時,發現皆可降低lovastatin誘導細胞凋亡現象發生。當lovastatin與Z-LEHD-fmk同時處理細胞時,可減少lovastatin所造成caspase 8活化。但當lovastatin與Z-IETD-fmk同時處理細胞時,無法顯著減少lovastatin造成的caspase 9活化。 可見得內生性凋亡路徑在lovastatin所導致之細胞凋亡扮演重要角色。 我們同時發現Lovastatin處理後, 可使p-ERK、p-p38及p-JNK的表現量上升。N-acetylcysteine 及MAPK pathway抑制劑,可降低lovastatin所導致之細胞凋亡反應。MAPK在lovastatin誘發細胞凋亡中扮演角色有待進一步研究。 | zh-TW |
dc.description.abstract | Oral cancer is the fifth leading cause of cancer-related deaths in male population in Taiwan. Despite recent advances in radiotherapy and chemotherapy, the survival of patients with oral cancer has not improved significantly. Continues investigation of new chemotherapeutic agents is need. The 3-hydroxy-3-methylglutaryl coenzymeA (HMG-CoA) reductase inhibitors, also known as statins, are commonly prescribed medications that lower serum cholesterol. Preclinical research performed during the past decade shows that statins have antineoplastic effects in many tumor cell lines. Therefore, we were interested in exploring the potential of lovastatin as an anti-cancer agent in oral cancer cells. We found lovastatin significantly inhibited the cell proliferation of Ca9-22 in a dose-dependent manner. Flow cytometric analysis of DNA content showed that lovastatin treatment induced apoptosis following G1 arrest. Western blotting showed lovastatin increased cytochrome c release and activated caspase-8 and caspase-9. Caspase-9 activation is earlier than caspase-8. The apoptosis could be inhibited by caspase 8 inhibitor (Z-LEHD-FMK) or caspase 9 inhibitor (Z-IETD-FMK). These results demonstrated that the intrinsic apoptotic pathway may play a major role in the lovastatin-induced apoptosis. Pretreatment of cells with N-acetyl cysteine (NAC), MAPKs inhibitors and PI3K/Akt inhibitors inhibited the PARP cleavage. MAPKs, PI3K/Akt pathway and ROS may play important roles in lovastatin-induced apoptosis. Taking together, the chemopreventive potential of lovastatin is required to be further determined with animal model and clinical trails in the future. | en |
dc.description.tableofcontents | 中文摘要 1bstract 2一章 導論 3-1 口腔癌 (Oral cancer) 3-2 細胞凋亡(Apoptosis) 4-3 細胞凋亡之分子機制 5-4 Bcl-2基因家族 8-5 TNF receptors 和death receptors 9-6 MAPK (Mitogen-activated protein kinase)pathway與細胞凋亡 11-7 內質網壓力(ER stress)與細胞凋亡 11-8 細胞週期 (Cell cycle) 12-9 Lovastatin藥物與其應用 13-10 Lovastatin藥理 14-11 Lovastatin之抗腫瘤機制 15二章 實驗方法與材料 17-1 細胞株培養 17-2 細胞存活率試驗 (MTT) assay 17-3 西方墨點法-細胞蛋白質的測定 18-4 Caspase抑制作用 20-5 其他抑制劑之抑制作用 20-6 分析細胞質內Cytochrome c 20-7 Cell death detection ELISA 21-8 利用Flow cytometry偵測細胞週期變化 21-9 利用TUNEL (Temianl deoxynucleotidyl Transferase-mediated dUTP –biotin nick end labeling)偵測細胞凋亡 22-10 Caspase Colorimetric Activity Assay 22三章 實驗結果 23-1、Lovastatin抑制Ca9-22細胞生長 23-2、Lovastatin誘導Ca9-22細胞凋亡反應 23-3、Lovastatin經由外在與內在凋亡路徑誘發口腔癌細胞凋亡 24-4、Lovastatin誘導內生性細胞凋亡 (intrinsic apoptotic pathway)機制 25-5、抑制死亡受體凋亡途徑對lovastatin誘導Ca9-22凋亡的影響 26-6、ROS (reactive oxygen species)、MAPK (Mitoge-Avtivated Protein Kinase) pathway及PI3K/Akt (Phosphatidylinositol/Akt) pathway對lovastatin誘導Ca9-22凋亡的影響 27-7、Lovastatin對於ER stress的影響 28四章 討論 29與附圖 34考文獻 64 | en |
dc.format | application/pdf | en |
dc.format.extent | 5940616 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | en |
dc.language.iso | en_US | - |
dc.subject | 口腔癌 | zh-TW |
dc.subject | lovastatin | zh-TW |
dc.subject | 細胞凋亡 | zh-TW |
dc.subject | Lovastatin | en |
dc.subject | Aapoptosis | en |
dc.subject | Oral cancer | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Lovastain誘導人類口腔癌細胞凋亡機轉之研究 | zh-TW |
dc.title | Study of Mechanism of Lovastatin-induced Apoptosis in Oral Cancer Ca9-22 cells. | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/178615/1/ntu-98-R96450004-1.pdf | - |
item.fulltext | with fulltext | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | open | - |
顯示於: | 口腔生物科學研究所
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