|Title:||Age-Dependent Increase in C7-1 Gene Expression in Rat Frontal Cortex||Authors:||HUNG, HUI-CHUAN
|Keywords:||C7-1;Regional distribution;Aging;V-ATPase;Frontal cortex||Issue Date:||2000||Journal Volume:||v.75||Journal Issue:||n.2||Start page/Pages:||330-336||Source:||MOLECULAR BRAIN RESEARCH||Abstract:||
We have previously reported that a fragment of mRNA, denoted as C7-1, which expression was significantly increased in the frontal cortex of aged rats. In the present study, we have cloned and sequenced the full length cDNA of the C7-1 gene. We have found that the open reading frame of this gene encoded a 463-amino-acid protein, which shared 84% identity in amino acid sequence with a subunit of vacuolar H+-ATPase (V-ATPase). Further Northern blot analysis revealed that there was an age-dependent increase in C7-1 gene expression in rat frontal cortex, but not in other brain areas. Moreover, application of C7-1 antisense oligonucleotide to cortical neuronal cultures markedly inhibited cell survival. These results together suggest that C7-1 is a marker for the aging process and that upregulation of C7-1 may be important in maintaining the normal function of V-ATPase during aging.cortical neuronal cultures markedly inhibited cell survival.
|Appears in Collections:||口腔生物科學研究所|
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