https://scholars.lib.ntu.edu.tw/handle/123456789/162656
標題: | Brain-Derived Neurotrophic Factor Antisense Oligonucleotide Impairs Memory Retention and Inhibits Long-Term Potentiation in Rats | 作者: | Ma, Y.L Wang, H.L Wu, H.C Wei, C.L Lee, E.H.Y |
關鍵字: | brain-derived neurotrophic factor;gene expression;memory retention;long-term potentiation;hippocampus | 公開日期: | 2000 | 卷: | v.82 | 期: | n.4 | 起(迄)頁: | 957-967 | 來源出版物: | NEUROSCIENCE | 摘要: | We have examined the relationship between brain-derived neurotrophic factor gene expression in the hippocampus and memory retention as well as long-term potentiation of rats. One-way inhibitory avoidance learning was adopted as the behavioural paradigm. Results revealed that brain-derived neurotrophic factor messenger RNA levels in the dentate gyrus of the hippocampus were markedly increased at 1 h, 3 h and 6 h post-training in rats showing good retention performance when compared with the poor retention controls. Direct injection of brain-derived neurotrophic factor antisense oligonucleotide into the dentate gyrus of the hippocampus before memory consolidation takes place markedly impaired retention performance in rats. It also significantly decreased brain-derived neurotrophic factor messenger RNA level in the dentate gyrus. The same antisense treatment also markedly reduced the amplitude and slope of excitatory postsynaptic potential as well as the brain- derived neurotrophic factor messenger RNA level in the dentate gyrus. These results suggest that hippocampal brain- derived neurotrophic factor gene expression plays an important role in the memory consolidation process and in the expression of long-term potentiation in rats. These results provide the first evidence to relate brain-derived neurotrophic factor gene expression and memory function in vertebrates. It further suggests that brain-derived neurotrophic factor gene expression is involved in behavioural plasticity. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/94754 |
顯示於: | 口腔生物科學研究所 |
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