https://scholars.lib.ntu.edu.tw/handle/123456789/162659
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 口腔生物研究所 | en |
dc.contributor.author | WU, HAN-CHUNG | en |
dc.contributor.author | LIN, CHIN-TARNG | en |
dc.creator | Wu, Han-Chung;Lin, Chin-Tarng | - |
dc.date | 2001 | en |
dc.date.accessioned | 2008-12-29T05:55:43Z | - |
dc.date.accessioned | 2018-07-09T05:28:14Z | - |
dc.date.available | 2008-12-29T05:55:43Z | - |
dc.date.available | 2018-07-09T05:28:14Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/94758 | - |
dc.description.abstract | The association of G protein beta2 subunit (G beta2) with mitotic spindles in various mammalian cells has been demonstrated previously. Recently, we have identified the association of G beta2 protein with microtubules (Wu et al., [1998] J. Cell. Biochem. 70: 552-562). In the present experiment we have demonstrated the possible functional role of G beta2 in microtubule and mitotic spindle organization in mammalian cells. When G beta2 antisense phosphorothioate oligonucleotides were transfected into mammalian cells, inhibition of cell proliferation with cell death after a 4- day treatment was observed. If the transfected cells were incubated for two days and their G beta2 and microtubules were examined by Western blotting and immunofluorescence localization, marked reduction of the G beta2 protein, fragmentation and disassembly of cytoplasmic microtubules, and disorganized mitotic spindles were found. We conclude that the G beta2 protein is closely associated with microtubule assembly and may play a potential role in the regulation of cell proliferation and microtubule and mitotic spindle organization in mammalian cells. J. Cell. Biochem. 83: 136-146, 2001. (C) 2001 Wiley-Liss, Inc. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | JOURNAL OF CELLULAR BIOCHEMISTRY v.83 n.1 pp.136-146 | en |
dc.relation.ispartof | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.subject | G beta 2 protein | en |
dc.subject | antisense oligonucleotide transfection | en |
dc.subject | microtubule assembly | en |
dc.subject | mitotic spindle organization | en |
dc.subject | nasopharyngeal carcinoma cells | en |
dc.subject | BINDING REGULATORY PROTEIN | en |
dc.title | G Protein Beta 2 Subunit Antisense Oligonucleotides Inhibit Cell Proliferation and Disorganize Microtubule and Mitotic Spindle Organization | en |
dc.relation.pages | 136-146 | - |
dc.relation.journalvolume | v.83 | - |
dc.relation.journalissue | n.1 | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | Pathology | - |
crisitem.author.orcid | 0000-0003-3928-1412 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 口腔生物科學研究所 |
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