|Title:||Identification of B-Cell Epitope of Dengue Virus Type 1 and Its Application in Diagnosis of Patients||Authors:||WU, HAN-CHUNG
|Keywords:||Dengue;Epitope;Neutralization;Phage display;Serologic Test;Taiwan||Issue Date:||2001||Journal Volume:||v.39||Journal Issue:||n.3||Start page/Pages:||977-982||Source:||JOURNAL OF CLINICAL MICROBIOLOGY||Abstract:||
Using a serotype-specific monoclonal antibody (MAb) of dengue virus type 1 (DEN-1), 15F3-1, we identified the B- cell epitope of DEN-1 from a random peptide library displayed on phage. Fourteen immunopositive phage clones that bound specifically to MAb 15F3-1 were selected. These phage- borne peptides had a consensus motif of HxYaWb (a = S/ T, b = K/H/R) that mimicked the sequence HKYSWK, which corresponded to amino acid residues 111 to 116 of the nonstructural protein 1 (NS1) of DEN-1. Among the four synthetic peptides corresponding to amino acid residues 110 to 117 of the NS1 of DEN-1, -2, -3, and -4, only one peptide , EHKYSWKS (P14M) of DEN-1, was found to bind to 15F3-1 specifically. Furthermore, P14M was shown to inhibit the binding of phage particles to 15F3-1 in a competitive inhibition assay. Histidine(111) (His(111)) was crucial to the binding of P14M to 15F3-1, since its binding activity dramatically reduced when it changed to leucine(111) (Leu( 111)). This epitope-based peptide demonstrated its clinic, EHKYSWKS (P14M) of DEN-1, was found to bind to 15 F3-1
|Appears in Collections:||口腔生物科學研究所|
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