|Title:||A Novel Peptide Specifically Binding to Nasopharyngeal Carcinoma for Targeted Drug Delivery||Authors:||李冬陽
LIN, CHIN -TARNG
|Keywords:||STERICALLY STABILIZED LIPOSOMES;PHAGE DISPLAY;SOLID TUMORS;MOUSE MODEL;THERAPEUTIC-EFFICACY;VESSEL LEAKINESS||Issue Date:||2004||Journal Volume:||v.64||Journal Issue:||n.21||Start page/Pages:||8002-8008||Source:||CANCER RESEARCH||Abstract:||
Nasopharyngeal carcinoma (NPC) is a common cancer among Chinese living in southern China, Taiwan, and Singapore. The 5-year survival rate in the early stage of NPC has been reported as high as 90 to 95% with the use of radiotherapy, but in the advanced cases, even with the use of both chemotherapy and radiotherapy, the survival rate is still < 50%. To improve the survival rate, we identify a 12-mer peptide (L-peptide) specifically binding to NPC cells with a phage displayed random peptide library. The L-pliage and synthetic L-peptide bound to the tumor cell surfaces of most NPC cell lines and biopsy specimens, but not normal nasal mucosal cells, and the L-peptide-linked liposomes containing fluorescent substance (L-peptide-Lipo-HPTS) were capable of binding to and translocating across plasma membranes. L- Peptide-linked liposomes that carried doxorubicin (L-peptide -Lipo-Dox) caused marked cytotoxicity in NPC cells. In SCID mice bearing NPC xenografts, the L-phages specifically bound to the tumor mass, an effect that was inhibited by competition with synthetic L-peptide. In addition, the L- peptide-Lipo-Dox suppressed tumor growth better than Lipo- Dox. These results indicate that the novel L- peptide specifically binds NPC cells and is a good candidate for targeted drug delivery to NPC solid tumors.
|Appears in Collections:||口腔生物科學研究所|
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