https://scholars.lib.ntu.edu.tw/handle/123456789/162870
標題: | Involvement of DNA-Dependent Protein Kinase in Normal Cell Cycle Progression through Mitosis | 作者: | Lee, Kyung-Jong Lin, Yu-Fen HAN-YI E. CHOU Yajima, Hirohiko Fattah, Kazi R. Lee, Sheng-Chung |
公開日期: | 2011 | 起(迄)頁: | 12796-12802 | 來源出版物: | Journal of Biological Chemistry | 摘要: | The catalytic subunit of DNA-dependent protein kinase DNA- PKcs plays an important role in DNA double-strand break DSB repair as the underlying mechanism of the non-homologous end joining pathway. When DSBs occur, DNA- PKcs is rapidly phosphorylated at both the Thr-2609 and Ser-2056 residues, and such phosphorylations are critical for DSB repair. In this study we report that, in addition to responding to DSBs , DNA-PKcs is activated and phosphorylated in normal cell cycle progression through mitosis. Mitotic induction of DNA- PKcs phosphorylation is closely associated with the spindle apparatus at centrosomes and kinetochores. Furthermore, depletion of DNA-PKcs protein levels or inhibition of DNA- PKcs kinase activity results in the delay of mitotic transition because of chromosome misalignment. These results demonstrate for the first time that DNA-PKcs, in addition to its role in DSB repair, is a critical regulator of mitosis and could modulate microtubule dynamics in chromosome segregation. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/240638 | DOI: | 10.1074/jbc.M110.212969 |
顯示於: | 口腔生物科學研究所 |
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