The acid waste solution was decanted and the crabs were rinsed with tap water.
The demineralized crabs were added into another 250ml glass flask containing 200ml
of 1N NaOH. After 30min of sonication, the flask was kept at 40oC for ca. 16hr.
After another 30min of sonication to disperse the solubilized materials, the alkaline
waste, principally the crab meat protein lysate, was decanted. The deproteinization
process was repeated about three times to obtain clear chitinous skins (or
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