https://scholars.lib.ntu.edu.tw/handle/123456789/176167
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 張森富 | en |
dc.contributor | 臺灣大學:生物產業機電工程學研究所 | zh_TW |
dc.contributor.author | 賴建洲 | zh |
dc.contributor.author | Lai, Chien-Chou | en |
dc.creator | 賴建洲 | zh |
dc.creator | Lai, Chien-Chou | en |
dc.date | 2004 | en |
dc.date.accessioned | 2007-11-26T05:41:44Z | - |
dc.date.accessioned | 2018-07-10T03:47:08Z | - |
dc.date.available | 2007-11-26T05:41:44Z | - |
dc.date.available | 2018-07-10T03:47:08Z | - |
dc.date.issued | 2004 | - |
dc.identifier | zh-TW | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/52921 | - |
dc.description.abstract | 摘要 針對康乃馨與玄蔘,使用不同瓶封口材質之研究證實提高組培瓶內、外氣體交換率(E),可解決芽體增殖時玻璃質化之問題,更能提高芽體生長之品質。玄蔘芽體增殖數目因E值提高而減少之問題,可透過啟動氣體交換之時機點來解決,例如使用4層藥包紙包覆組織培養瓶口,再以2層石蠟膜(Parafilm)緊密封住瓶口的栽培模式中,於4週後將石蠟膜移除,如此可以產生無玻璃質化、高品質、高數目之芽體。 除了培養基調製、裝填與殺菌作業之機械化、自動化及配合移植作業之生產動線安排之外,廠內使用活動式多層立體床架可提高單位土地面積上之種植面積達13倍,床架配備往復式自走光照裝置更可降低一半之光照設備成本。低成本之風扇水牆設施不但可降低培養室內溫度,更可提高組培瓶內外氣體交換率。一間具高產能、低成本的組織培養苗量產植物工廠已建構完成,正進行商業量產中。 高亮度發光二極體作為組培苗人工光源具發展潛力,交流供電全紅光適用於彩色海芋(Black Magic)組培苗栽培。 關鍵詞:植物工廠、組織培養、發光二極體 | zh_TW |
dc.description.abstract | Abstract Increasing gas exchange rate (E) of the TC vessel can reduce the vitrification problem frequently found in carnation (Dianthus caryophllus L. cv. Opale) shoot tip culture and adventitious buds production of Scrophularia yoshimurae. More over, higher quality of carnation shoot tip can be produced by increasing E value was found. Problem of decreasing buds counts when increasing E value can be solved by the control on the timing to initiate the gas exchange process. When 4 layers of dispense paper covered with 2 layers of parafilm was used, the highest buds count and best quality buds with no vitrification occurred can be derived if the timing to remove the parafilm to initiate the gas exchange process is set at the 4th week after transplanting. Besides the design and installation of the transplanting room and the production line for the preparation of cultural media, filling, and sterilization of TC vessels, a 2 floors production building has been established. Within the building, movable multi-layer benches equipped with retractable light mounting fixture were installed which can increase 13 times planting area per unit land area and cost down half of the light. Fan and pad system was used to not only remove the extra thermal energy but also increase the exchange rate of gas inside and outside vessels. A high productivity and low cost plant factory for the mass production of tissue culture (TC) plantlets has been established and is currently under commercial operations. High intensity light-emitting diode (LED) is the promising light source for TC operations. Providing with alternate current (AC), red LEDs alone can be used to grow TC plantlets of Calla Lily (Black Magic). KEYWORD: Plant Factory, Tissue Culture, LED | en |
dc.description.tableofcontents | 目 錄 誌謝-----------------------------------------i 中文摘要------------------------------------ii 英文摘要-----------------------------------iii 目錄-----------------------------------------v 表目錄-------------------------------------vii 圖目錄------------------------------------viii 第一章 前言--------------------------------1 第二章 文獻探討----------------------------4 2-1 溫度----------------------------------6 2-2 水牆風扇降溫系統------------------------7 2-3 光------------------------------------9 2-4 發光二極體應用於組培苗栽培與彩色海芋—9 2-5 氣體交換率---------------------------12 2-6 二氧化碳濃度-------------------------18 2-7 植物工廠-----------------------------19 第三章 試驗設備與方法---------------------20 3-1 瓶內外氣體交換率之量測---------------20 3-2 培養基組成與培養容器覆蓋物對康乃馨組 織培養苗玻璃質化與發根的影響---------22 3-3 容器覆蓋物(換氣)對玄蔘組織培養苗玻璃質化 之影響-------------------------------24 3-4 建構組織培養苗量產之植物工廠---------25 3-5 紅光LED應用於彩色海芋組培苗栽培---------38 第四章 結果與討論-------------------------42 4-1 不同瓶口覆蓋物之氣體交換率-----------42 4-2 瓶封口材料、培養基成份及處理方法對康 乃馨瓶苗之影響-----------------------45 4-3 瓶封口材料、培養基成份及處理方法對玄 蔘瓶苗之影響-------------------------53 4-4 組織培養苗量產植物工廠之建構---------61 4-5 紅光發光二極體(LED) 應用於彩色海芋組培苗 栽培--------------------------------------69 第五章 結論-------------------------------83 參考文獻-----------------------------------85 附錄一、立體人工環境栽培室中華民國專利證書-96 附錄二、立體人工環境栽培室美國專利證書-----97 附錄三、全自動往復移動式多段供料暨均風系統 中華民國專利證書-------------------98 附錄四、全自動往復移動式多段供料暨均風系統 美國專利證書-----------------------99 | zh_TW |
dc.language | zh-TW | en |
dc.language.iso | en_US | - |
dc.subject | 植物工廠 | en |
dc.subject | 組織培養 | en |
dc.subject | 發光二極體 | en |
dc.subject | Plant Factory | en |
dc.subject | Tissue Culture | en |
dc.subject | LED | en |
dc.title | 建構組織培養苗量產植物工廠之研發 | zh |
dc.title | Development of a Plant Factory for the Mass Production of Tissue Culture Plantlets In Vitro | en |
dc.type | thesis | en |
dc.relation.reference | 參考文獻 方煒。1994。水牆設計與使用。農業機械學刊 3(4): 57-70。 方煒。1998。植物工廠。種苗生產自動化技術通訊。 第三期第98001號。 種苗生產自動化技術服務團。台北:財團法人農業機械化研究發展中心。 方煒。2003。溫室蒸發冷卻降溫系統設計與模擬軟體之建立。2003資訊科技在農業之應用研討會,12月 1-2日,台北。中華民國。 方煒、賴建洲 2001. 植物量產工程在台灣的實現。生物科技在農業上的應用研討會論文集。楊盛行主編。台灣大學。ISBN 957-02-9599-6。pp295-311. 古新梅、蔡新聲。1994a。培養基組成分對康乃馨試管苗玻璃質化的影響。中華農業研究 43(1) : 51-62。 古新梅、蔡新聲。1994b。繼代培養對康乃馨試管苗玻璃質化的影響。中華農業研究 43(3) : 308-319。 沈再發。2000。海芋栽培技術手冊。種苗改良繁殖場出版。 賴建洲。1999。立體人工環境栽培室。中華民國新型專利 398178。中華民國。 賴建洲。1999。全自動往復移動式多段供料與均風系統。中華民國新型專利 431129。中華民國。 賴建洲、李文台、張耕鵬、方煒。2001。彩色海芋健康種球快速量產與高產高品質切花生產技術之開發。農業生物技術國家型科技計畫構想書。中華民國。 Adkins, S.W. 1992. Cereal callus cultures: control of headspace gases can optimize the conditions for callus proliferation. Aust. J. Bot. 40: 737-749 Aguilar, M.L., F.L. Espadas, J. Coello, B.E. Maust, C. Trejo, M.L. Robert and J.M. Santamaria. 2000. The role of abscisic acid in controlling leaf water loss, survival and growth of micropropagated Tagetes erecta plants when transferred directly to the field. J. Exp. Bot. 51: 1861-1866 Armstrong, J., E.E.P. Lemos, S.M.A. Zobayed, S.H.F.W. Justin, and W. Armstrong. 1997. A humidity-induced convective through flow ventilation system benefits Annona squamosa L. explants and coconut colloid. Ann. Bot. 79: 31-40 Ando, T. 1978. Gaseous environment in the airtight culture vessel containing orchids. Abst. Annual Autumn Meet. Jap. Soc. Hort.Sci., pp. 368-369. Appelgren, M. 1991. Effects of light quality on stem elongation of Pelargonium in vitro. Scientia Horticulture 45:345-351. Aksenova, N.P., T.N. Konstantinova, L.I. Sergeeva, I. Machackova and S.A. Golyanovskaya. 1994. Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones. J. Plant Growth Regul. 13:143-146. Brown, C.S., A.C. Schuerger and J.C. Sager. 1995. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J. Amer. Soc. Hort. Sci. 120:808-813. Bula, R.J., R.C. Morrow, T.W. Tibbitts, D.J. Barta, R.W. Ignatus and T.S. Martin. 1991. Light-emitting diodes as a radiation source for plants. HortScience 26:203-205. Chiu, N.Y. and K.H. Chang. 1998. The illustrated medicinal plants of Taiwan. SMS Publ Inc, Taipei, Taiwan V5: p 194. Debergh, P.C. and L. Maene. 1984. Pathological and physiological problems related to the in vitro cultures of plants. Parasitica 40: 69-75. Debergh, P., J. Aitken-Christie, D. Cohen, B. Grout, S. von Arnold, R. Zimmerman, and M. Ziv. 1992. Reconsideration of the term vitrification as used in micropropagation. Plant Cell Tissue Organ Cult. 30: 135-140 Desjardins, Y., F. Laforge, C. Lussier and A. Gosselin. 1988. Effect of CO2 enrichment and high photosynthetic photon flux on the development of autotropy and growth of tissue culture strawberry, raspberry and asparagus plants. Acta Hort. 230:45-53. Dooley, J.H. 1991. Influence of lighting spectra on plant tissue culture, ASAE paper No. 917530, Amer. Soc. Agr. Eng., St. Joseph, Ml, USA. Economou, A. S. and P. E. Read. 1987. Light treatments to improve efficiency of in vitro propagation system. HortScience 22(5):751-754. Fang, W. and R. C. Jao. 2000. A review on artificial lighting of tissue cultures and transplants. p. 108-113 In: C. Kubota and C. Chun (eds.), Transplant Production in the 21st Century. Kluwer Academic Publishers. Netherlands. Fujiwara, K., T. Kozai and I. Watanabe. 1987. Fundamental studies on environment in plant tissue culture vessels. J. Agric. Meteorol. 43:21-30. Gould, J. H. and T. Murashige. 1985. Morphogenic substances released by plant tissue cultures. I. Identification of berberine in Nandina culture medium, morphogenesis, and factors influencing accumulation. Plant Cell Tissue Organ Cult 4: 29-42. Grodzinski, B., I. Boesel and K. Horton. 1981. Effect of light and carbon dioxide on release of ethylene from leaves of Xanthium strumarium. Plant Physiol 67: 272-273. Hakkart, F.A. and J.A. Versluijs. 1983. Some factors affecting glassiness in carnation meristem tip cultures. Neth. J. Path. 89:47-53 Infante, R., E. Magnanini and B. Righetti. 1989. The role of light and CO2 in optimizing the conditions for shoot proliferation of Actinidia deliciosa in vitro. Physiol. plant 77:191-195. Iwanami, Y., T. Kozai, Y. Kitaya and S. Kino. 1992. Effects of supplemental red and far-red lighting using light emitting diode on stem elongation and growth of potato plantlets in vitro. p. 183. In Abstr. Intl. Symp. Transplant Production Sytems. 21-26 July 1992. Yokohama Japan Jao, R.C. and W. Fang. 2003. An adjustable light source for photo-phyto related research and young plant production. Appl. Eng. Agric. 19(5):601-608. Jao, R.C. and W. Fang. 2004a. Effects of frequency and duty ratio on the growth of potato plantlets in vitro using light emitting diodes. HortScience 39(2):375-379. Jao, R.C. and W. Fang. 2004b. Growth of potato plantlets in vitro is different when provided concurrent versus alternating red and blue light photoperiods. HortScience 39(2):380-382. Jo, M. H., I. K. Ham, A. M. Lee, M. E. Lee, H. N. Song, H. G. Han, and S. I. Woo. 2002 Effect of sealing materials and photosynthetic photon flux of culture vessel on growth and vitrification in carnation plantlets in vitro. J. Korean Soc. Hort. Sci. 43: 133-136. Kevers, C., M. Coumans, M. F. Coumans-Gilles and T. Gasper. 1984. Physiological and biochemical events leading to vitrification of plants cultured in vitro. Physiol. Plant. 61: 69-74. Kevers, C., N. Boyer, J.C. Courduroux and T. Gasper. 1992. The influence of ethylene on proliferation and growth of rose shoot cultures. Plant Cell Tissue Organ Cult. 28: 175-181. Kozai, T., K. Fujiwara and I. Wanatabe. 1986. Fundamental studies on environments in plant tissue culture vessels: effect of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J. Agr. Meteorol. 42: 119-127 Kozai, T., M. Hayashi and M. Ochiai. 1991. Effect of sideward lighting on the growth and morphogenesis of potato plantlets in vitro. J. Jap. Soc. Hort. Sci. 60.: 228-229. Kozai, T. and K. Sekimoto. 1988. Effects of the number of air changes per hour of the closed vessel and photosythetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry plantlets in vitro. Environ. Control Biol. 26:21-29. Kozai T, and M. A. L. Smith. 1995. Environmental control in plant tissue culture. In: Aitken-Christie J, Kozai T, Smith MAL (eds.) Automation and Environmental Control in Plant Tissue Culture. Kluwer, Dordrecht, pp 301-318. Kumar PP, Reid DM, Thorpe TA (1987) The role of ethylene and carbon dioxide in differentiation of shoot buds in excised cotyledons of Pinus radiata in vitro. Physiol Plant 69: 244-252 Lai, C. C., 2001. Artificial cultivating room and method for cultivating plants. US Patent 6279263 B1. Lai, C. C., 2003. Automatically reciprocal multi-stage supply and ventilation system. US Patent 66666391B2. Lai, C. C., W. Fang and S.F. Chang. 2001. Plant Factory in Taiwan Using Moving Light. Proceedings of "International Symposium on Design and Environmental Control of Tropical and Subtropical Greenhouses". April 15-18, Taichung, Taiwan, ROC. Majada, J. P., M. I. Sierra and R. Sanchez-Tames. 2001. Air exchange rate affects the in vitro developed leaf cuticle of carnation. Sci. Hort. 87: 121-130 Miyashita, Y., T. Kimura, Y. Kitaya and T. Kozai. 1994. Effects of red light on the growth and morphology of potato plantlets in vitro: using light emitting diodes (LEDs) as light source for micropropagation. Acta Horticulture 418:169-173. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497. Pâques, M. and P. Boxus. 1987. “Vitrification” : review of literature. Acta Horti. 212 : 155-166. Pâques, M. 1991. Vitrification and micropropagation : causes, remedies and prospects. Acta Horti. 289:283-290. Park, S. W., J. H. Jeon, S. H. Kim, Y. M. Park, C. Aswath and H. Joung. 2004. Effect of sealed and vented gaseous microenvironment on hyperhydricity of potato shoots in vitro. Sci. Hort. 99: 199-205. Pospisilova, J., J. Catsky, J. Solarova and I. Ticha. 1988. Photosynthesis of plant regenerates. Specificity of in vitro conditions and plantlets response. Biol. Plant.29:415-421. Read, P. E. 1990. Environmental effects in micropropagation. In: Handbook of plant cell culture, Vol.5. (eds). Ammirato, P.V. et al., pp. 95-125. McGraw-Hill Pub., New York. Sagare, A. P., C. L. Kuo, F. S. Chueh and H. S. Tsay 2001. De novo regeneration of Scrophularia yoshimurae YAMAZAKI (Scrophulariaceae) and quantitative analysis of harpagoside, an iridoid glucoside, formed in aerial and underground parts of in vitro propagated and wild plants by HPLC. Biol. Pharm. Bull. 24: 1311-1315. Searook, J. E. A. 1987. Changing the growth and morphology of potato plantlets in vitro by varying the illumination source. Acta. Hort. 212:401-410. Taiz, L. and E. Zeiger. 1991. Plant Physiology. 1st ed., 179-264. New York: Benjamin/Cummings Publishing Company, Inc. Tsay, H. S. 1998. Effects of medium composition at different recultures on vitrification of carnation (Dianthus caryophyllus) in vitro shoot proliferation. Acta Hort. 461: 243-249. Ziv, M. 1986. In vitro hardening and acclimatization of tissue culture plants. pp. 187-196. In : Plant Tissue Culture and its Agriculture Applications. (Eds. by Withers, L. A. and P. G. Alderson.). Butterworths London Ziv, M. 1991. Vitrification : morphological and physiological disorders of in virto plants. In: Micropropagation. (eds. P.C. Debergh and R.H. Zimmerman). pp. 45-69. Kluwer Academic Publishers. Zobayed, S.M.A., J. Armstrong and W. Armstrong. 1999. Cauliflower shoot-culture: effect of different types of ventilation on growth and physiology. Plant Sci. 141: 209-217 Zobayed, S.M.A., F. Afreen and T. Kozai. 2000. Quality biomass production via photoautotrophic micropropagation. Acta Hort. 530:377-386 Zobayed, S.M.A., J. Armstrong and W. Armstrong. 2001a. Leaf anatomy of in vitro tobacco and cauliflower plantlets as affected by different types of ventilation. Plant Sci. 161: 537-548 Zobayed, S.M.A., J. Armstrong and W. Armstrong. 2001b. Micropropagation of potato: evaluation of closed, diffusive and forced ventilation on growth and tuberization. Ann Bot. 87: 53-59 Zobayed, S.M.A., J. Armstrong, W. Armstrong. 2002. Multiple shoot induction and leaf and flower bud abscission of Annona cultures as affected by types of ventilation. Plant Cell Tiss. Org. Cult. 69: 155-165 | zh_TW |
item.languageiso639-1 | en_US | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_46ec | - |
item.openairetype | thesis | - |
item.grantfulltext | none | - |
顯示於: | 生物機電工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。