https://scholars.lib.ntu.edu.tw/handle/123456789/176370
標題: | Real-time assay of immobilized tannase with a stopped-flow conductometric device | 作者: | Lu, Fu-Ming Chang, Fu-Shiang Chen, Po-Chung Chen, L.C. Richie TZONG-JIH CHENG |
關鍵字: | Conductometric;Enzyme kinetics;Tannic acid;Stopped-flow | 公開日期: | 7-二月-2006 | 出版社: | Taipei:National Taiwan University Coll Management | 起(迄)頁: | - | 來源出版物: | Bioelectrochemistry 69, 113–116 | 摘要: | A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant=103.2 cm−1) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/2006111501264990 | 其他識別: | 246246/2006111501264990 | DOI: | 10.1016/j.bioelechem.2005.12.004 |
顯示於: | 生物機電工程學系 |
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