|Title:||Carbon Nanoparticle-Enhanced Immunoelectrochemical Detection for Protein Tumor Marker with Cadmium Sulfide Biotracers||Authors:||Ho, Ja-an Annie
|Issue Date:||2009||Journal Volume:||81||Journal Issue:||4||Start page/Pages:||1340-1346||Source:||Analytical Chemistry||Abstract:||
We have developed a sensitive electrochemical immu-noassay system for the detection of a protein tumor marker, carcinoembryonic antigen (CEA), that is based on a carbon nanoparticle (CNP)/poly(ethylene imine) (PEI)-modified screen-printed graphite electrode (CNP-PEI/SPGE) covered with anti-CEA antibodies. The signal amplification strategy - using CdS nanocrystals as biotrac-ers and CNPs to enhance electron transfer - improves the sensitivity and detection limit for CEA, suggesting that this system holds promise for development into a point-of-care or disposable home-care self-diagnostic tool. This biosensor is based on a sandwich complex immunoassay, which we assembled from sequential layers of the anti-CEA antibody (αCEA) on CNP-PEI/SPGE, the CEA sample, and the CdS nanocrystal quantum dots (QDs) sensitized with αCEA (αCEA-CdS QD). We used square wave anodic stripping voltammetry (SWASV) to amplify the signal current response obtained from the dissolved rCEA-CdS QDs. The calibration curve for CEA concentration was linear in the range of 0.032-10 ng/mL; the detection limit (estimated as the mean of the blank sample plus three times the standard deviation obtained on the blank sample) was 32 pg/mL (equivalent to 160 fg in a 5 μL sample). This method is suitably precise and sensitive to function as a means of determining urinary CEA, which is a better marker than serum CEA for the early detection of urothelial carcinoma. ? 2009 American Chemical Society.
|DOI:||10.1021/ac801832h||metadata.dc.subject.other:||A carbons; Biotracers; Cadmium sulfides; Calibration curves; Carbon nanoparticles; Carcinoembryonic antigens; Cds; CdS nanocrystals; Detection limits; Diagnostic tools; Early detections; Electron transfers; Point of cares; Polyethylene imines; Sandwich complexes; Signal amplifications; Signal currents; Square wave anodic stripping voltammetries; Standard deviations; Tumor markers; Urothelial carcinomata; Amplification; Antibodies; Antigens; Bioassay; Biosensors; Cadmium; Cadmium compounds; Data storage equipment; Dissolution; Ethylene; Graphite; Graphite electrodes; Highway planning; Nanocrystals; Nanoparticles; Nitrogen compounds; Signal processing; Strategic planning; Stripping (dyes); Tumors; Semiconductor quantum dots; cadmium sulfide; carbon; carcinoembryonic antigen; imine; nanocrystal; nanoparticle; poly(ethylene imine); unclassified drug; cadmium derivative; cadmium sulfide; carcinoembryonic antigen; graphite; immobilized antibody; polyethyleneimine; quantum dot; sulfide; tumor marker; article; biosensor; calibration; controlled study; disease marker; disposable equipment; electrochemical analysis; electrode; electron transport; home care; human; immunoassay; potentiometry; protein immobilization; self evaluation; urogenital tract cancer; chemistry; electrochemistry; immunology; methodology; Antibodies, Immobilized; Cadmium Compounds; Calibration; Carbon; Carcinoembryonic Antigen; Electrochemistry; Electrodes; Graphite; Humans; Immunoassay; Nanoparticles; Polyethyleneimine; Quantum Dots; Sulfides; Tumor Markers, Biological
|Appears in Collections:||化學系|
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