https://scholars.lib.ntu.edu.tw/handle/123456789/181192
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Ho, Ja-an Annie | en |
dc.contributor.author | Lin, Yeh-Chun | en |
dc.contributor.author | Wang, Li-Sheng | en |
dc.contributor.author | Hwang, Kuo-Chu | en |
dc.contributor.author | Chou, Pi-Tai | en |
dc.creator | Ho, Ja-An Annie; Lin, Yeh-Chun; Wang, Li-Sheng; Hwang, Kuo-Chu; Chou, Pi-Tai | - |
dc.date | 2009 | en |
dc.date.accessioned | 2009-09-09T11:08:31Z | - |
dc.date.accessioned | 2018-07-10T08:00:34Z | - |
dc.date.available | 2009-09-09T11:08:31Z | - |
dc.date.available | 2018-07-10T08:00:34Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/165041 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw/bitstream/246246/165041/1/142.pdf | - |
dc.description.abstract | We have developed a sensitive electrochemical immu-noassay system for the detection of a protein tumor marker, carcinoembryonic antigen (CEA), that is based on a carbon nanoparticle (CNP)/poly(ethylene imine) (PEI)-modified screen-printed graphite electrode (CNP-PEI/SPGE) covered with anti-CEA antibodies. The signal amplification strategy - using CdS nanocrystals as biotrac-ers and CNPs to enhance electron transfer - improves the sensitivity and detection limit for CEA, suggesting that this system holds promise for development into a point-of-care or disposable home-care self-diagnostic tool. This biosensor is based on a sandwich complex immunoassay, which we assembled from sequential layers of the anti-CEA antibody (αCEA) on CNP-PEI/SPGE, the CEA sample, and the CdS nanocrystal quantum dots (QDs) sensitized with αCEA (αCEA-CdS QD). We used square wave anodic stripping voltammetry (SWASV) to amplify the signal current response obtained from the dissolved rCEA-CdS QDs. The calibration curve for CEA concentration was linear in the range of 0.032-10 ng/mL; the detection limit (estimated as the mean of the blank sample plus three times the standard deviation obtained on the blank sample) was 32 pg/mL (equivalent to 160 fg in a 5 μL sample). This method is suitably precise and sensitive to function as a means of determining urinary CEA, which is a better marker than serum CEA for the early detection of urothelial carcinoma. ? 2009 American Chemical Society. | - |
dc.format | application/pdf | en |
dc.format.extent | 1618520 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | en | en |
dc.language.iso | en_US | - |
dc.relation | Analytical Chemistry 81 (4): 1340-1346 | en |
dc.relation.ispartof | Analytical Chemistry | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | A carbons; Biotracers; Cadmium sulfides; Calibration curves; Carbon nanoparticles; Carcinoembryonic antigens; Cds; CdS nanocrystals; Detection limits; Diagnostic tools; Early detections; Electron transfers; Point of cares; Polyethylene imines; Sandwich complexes; Signal amplifications; Signal currents; Square wave anodic stripping voltammetries; Standard deviations; Tumor markers; Urothelial carcinomata; Amplification; Antibodies; Antigens; Bioassay; Biosensors; Cadmium; Cadmium compounds; Data storage equipment; Dissolution; Ethylene; Graphite; Graphite electrodes; Highway planning; Nanocrystals; Nanoparticles; Nitrogen compounds; Signal processing; Strategic planning; Stripping (dyes); Tumors; Semiconductor quantum dots; cadmium sulfide; carbon; carcinoembryonic antigen; imine; nanocrystal; nanoparticle; poly(ethylene imine); unclassified drug; cadmium derivative; cadmium sulfide; carcinoembryonic antigen; graphite; immobilized antibody; polyethyleneimine; quantum dot; sulfide; tumor marker; article; biosensor; calibration; controlled study; disease marker; disposable equipment; electrochemical analysis; electrode; electron transport; home care; human; immunoassay; potentiometry; protein immobilization; self evaluation; urogenital tract cancer; chemistry; electrochemistry; immunology; methodology; Antibodies, Immobilized; Cadmium Compounds; Calibration; Carbon; Carcinoembryonic Antigen; Electrochemistry; Electrodes; Graphite; Humans; Immunoassay; Nanoparticles; Polyethyleneimine; Quantum Dots; Sulfides; Tumor Markers, Biological | - |
dc.title | Carbon Nanoparticle-Enhanced Immunoelectrochemical Detection for Protein Tumor Marker with Cadmium Sulfide Biotracers | en |
dc.type | journal article | en |
dc.identifier.doi | 10.1021/ac801832h | - |
dc.identifier.scopus | 2-s2.0-63649084071 | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
dc.relation.pages | 1340-1346 | - |
dc.relation.journalvolume | 81 | - |
dc.relation.journalissue | 4 | - |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/165041/1/142.pdf | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
crisitem.author.dept | Biochemical Science and Technology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Professional Master's Program of Biotechnology Management(PMBM) | - |
crisitem.author.dept | College of Life Science | - |
crisitem.author.dept | Applied Mechanics | - |
crisitem.author.dept | Chemistry | - |
crisitem.author.dept | Center for Emerging Material and Advanced Devices | - |
crisitem.author.orcid | 0000-0001-6684-1118 | - |
crisitem.author.orcid | 0000-0003-1512-9109 | - |
crisitem.author.orcid | 0000-0002-8925-7747 | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | School of Professional Education & Continuing Studies(SPECS) | - |
crisitem.author.parentorg | National Taiwan University | - |
crisitem.author.parentorg | College of Engineering | - |
crisitem.author.parentorg | College of Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
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