https://scholars.lib.ntu.edu.tw/handle/123456789/181293
標題: | Bioconjugated Gold Nanodots and Nanoparticles for Protein Assays Based on Photoluminescence Quenching | 作者: | Huang, Chih-Ching Chiang, Cheng-Kang ZONG-HONG LIN Lee, Kun-Hong HUAN-TSUNG CHANG |
公開日期: | 2008 | 卷: | 80 | 期: | 5 | 起(迄)頁: | 1497-1504 | 來源出版物: | Analytical Chemistry | 摘要: | This study describes the first instance of the use of two differently sized Au nanoparticles (Au NPs), acting separately as donor and acceptor, in homogeneous photoluminescence quenching assays developed for the analysis of proteins. Introduction of a breast cancer marker protein, platelet-derived growth factor AA (PDGF AA), to a solution of 11-mercaptoundecanoic acid-protected, 2.0-nm photoluminescent Au nanodots (LAuND) led to the preparation of PDGF AA-LAuND as the donor. Thiol-derivative PDGF binding aptamers (Apt) and 13-nm spherical Au NPs were used to synthesize the Apt-QAuNP acceptor. The photoluminescence of PDGF AA-LAuND at 520 nm decreased when photoluminescence quenching occurred between Apt-QAuNP and PDGF AA-LAuND. We used the PDGF AA-L AuND/Apt-QAuNP-based molecular light switching system to analyze PDGFs and PDGF α-receptor in separate homogeneous solutions. In the presence of PDGFs, the interaction between Apt-QAuNP and PDGF AA-LAuND decreased as a result of competitive reactions between the PDGFs and Apt-QAuNP. Similarly, the interaction between Apt-Q AuNP and PDGF AA-LAuND reduced as a result of competitive reactions between PDGF α-receptor and PDGF AA-LAuND. The limits of detection (LODs) for PDGF AA and PDGF α-receptor were 80 pM and 0.25 nM, respectively, resulting from a low background photoluminescence signal. When using the Apt-QAuNP as selectors for (a) the enrichment of PDGF AA and (b) the removal of matrixes possessing intense background fluorescence from cell media and urine samples, the LOD for PDGF AA decreased to 10 pM. Unlike quantum dots, the LAuND provide the advantages of biocompatibility, ease of bioconjugation, and minimal toxicity. ? 2008 American Chemical Society. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/165279 http://ntur.lib.ntu.edu.tw/bitstream/246246/165279/1/101.pdf |
DOI: | 10.1021/ac701998f | SDG/關鍵字: | Binding aptamers; Breast cancer; Molecular light switching system; Photoluminescence quenching assays; Assays; Gold compounds; Nanoparticles; Photoluminescence; Proteins; Quenching; Semiconductor quantum dots; 11 mercaptoundecanoic acid; aptamer; biological marker; gold derivative; nanoparticle; platelet derived growth factor; platelet derived growth factor AA; platelet derived growth factor alpha receptor; quantum dot; thiol derivative; unclassified drug; undecanoic acid; article; binding assay; binding competition; biocompatibility; breast cancer; conjugation; controlled study; disease marker; human; human cell; light; normal human; photochemical quenching; photoluminescence; protein analysis; protein binding; protein determination; protein interaction; synthesis; toxicity; urinalysis; Cell Line, Tumor; Gold; Humans; Luminescence; Nanostructures; Optics; Platelet-Derived Growth Factor; Receptors, Platelet-Derived Growth Factor; Thrombin |
顯示於: | 化學系 |
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