|Title:||Albumin Messenger Rna Is Not Specific for Circulating Hepatoma Cells||Authors:||CHOU, HUEI-CHI
|Issue Date:||1994||Journal Volume:||v.107||Journal Issue:||n.2||Start page/Pages:||630-631||Source:||GASTROENTEROLOGY||Abstract:||
In the January 1994 issue of Gastroneterology, Hillaire et al.1 and Carr2 discussed in length that detection of albumin messenger RNA (mRNA) by reverse transcription- polymerase chain reaction (RT-PCR) in the blood could be used as a marker of circulating hepatocellular carvinoma ( HCC) cells. However, we have found that albumin mRNA can be detected in most normal subjects in their peripheral blood and is not suitable for this purpose. In Taiwan, HCC is the leading cause of cancer death. Recurrent HCC after resection or distant HCC metastasis is frequently found.3 Detection of cireulating micrometastasis is important for the understanding of the biological behavior of HCC and the teeatment plan. Detection of tissue-specific mRNA by RT-PCR as a marker of hematogenous micrometastasis has been described in melanoma, neuroblastoma, and prostate cancer.4- 6 In the past year, we have tried to find a sensitive and specific marker that can be used as an indicator of the presence of circulating HCC cells. Because albumin was generally thought to be exclusively expressed in the liver7 and hepatocyte is normally not found in the blood, we used RT-PCR to detect the albumin mRNA as a marker of the presence of HCC cells in the circulation. First we tested whether the albumin mRNA would be detected in the peripheral blood of a normal subject . The blood cell pellets were obtained from noncoagulated blood after venipuncture. T otal RNA was extracted from the pellets as previously described. 8 One microgram of total RNA was reverse transcribed to complementary DNA. Five microliters of the total 20 μL complementary DNA product was subdected to nested PCR. The primer for the first PCR was primer AL64S20: 5' GCC TTT GGC ACA ATG AAG TG 3' in exon 1 and primer AL 682R20: 5' ACA GGC AGG CAG CTT TAT CA 3' in exon 5. The primer for second PCR was primer AL113S20: 5' TTA GCT CGG CTT ATT CCA GG 3' in exon 1 and primer AL 518R20: 5' CAC ATC ACA TCA ACC TCT GG 3 ' in exon 4.9 The condition for PCR was as previously described.8 To our surprise, we found that the specific band of 406 base pairs of albumin mRNA with a size of 406 base pairs could be found in 6 of 7 normal subjects. The experiments were repeated several times, and the results were reproducible. In addition, 12 of 14 patients with HCC
|Appears in Collections:||醫學系|
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