https://scholars.lib.ntu.edu.tw/handle/123456789/188067
標題: | Oxidized Low-Density Lipoproteins Inhibit Endothelial Cell Proliferation by Suppressing Basic Fibroblast Growth Factor Expression | 作者: | Chen, C.-H. Jiang, W. Via, D. P. Luo, S. Li, T.-R. Lee, Y.-T. Henry, P. D. |
關鍵字: | lipoproteins;growth substances;endothelium;genes;angiogenesis;PLATELET-ACTIVATING-FACTOR | 公開日期: | 2000 | 卷: | v.101 | 期: | n.2 | 起(迄)頁: | 171-177 | 來源出版物: | CIRCULATION | 摘要: | Background-Hyperlipidemia inhibits proliferation of endothelial cells ( ECs) in culture and angiogenesis in vivo and in arterial explants. Elucidation of the mechanisms may suggest novel therapies against atherosclerosis. Methods and Results-Basic Fibroblast growth factor (bFGF) expression and mitogenic effects were assessed in bovine aortic ECs incubated with oxidized LDL (ox-LDL). Compared with native LDL and Lipoprotein-free controls, ox-LDL reduced bFGF mRNA levels in a time- and concentration-dependent manner, 100 mu g/mL producing a maximum reduction of 40% to 50% within 24 to 48 hours. There were commensurate reductions in intracellular and extracellular bFGF concentrations, DNA and total RNA syntheses, and cell replication. FGF receptor 1 and beta-actin mRNA levels were unchanged. Ox-LDL accelerated bFGF mRNA degradation in actinomycin D -treated cells. However, inhibition of bFGF expression by ox-LDL was attenuated by cyclohexamide, indicating a requirement for continuous new protein synthesis for posttranscriptional destabilization, Reduced syntheses of DNA and total RNA were completely restored by bFGF but not by vascular endothelial growth factor. Inhibition of total RNA synthesis achieved by exposing cells to a bFGF-neutralizing antibody was similar in magnitude: to that induced by ox-LDL. Conclusions -Cytotoxic effects of ox -LDL on ECs are attributable in part to suppression of bFGF expression. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95111 |
顯示於: | 醫學系 |
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