https://scholars.lib.ntu.edu.tw/handle/123456789/188113
DC Field | Value | Language |
---|---|---|
dc.contributor | 內科 | en |
dc.contributor.author | CHEN, DING-SHINN | en |
dc.contributor.author | 陳培哲 | zh-tw |
dc.creator | 陳定信;陳培哲 | zh-tw |
dc.creator | CHEN, DING-SHINN;CHEN, PEI-JER | en |
dc.date | 2001 | en |
dc.date.accessioned | 2008-12-29T07:08:31Z | - |
dc.date.accessioned | 2018-07-11T05:22:22Z | - |
dc.date.available | 2008-12-29T07:08:31Z | - |
dc.date.available | 2018-07-11T05:22:22Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/95160 | - |
dc.description.abstract | Hepatitis delta virus (HDV) small delta antigen (S-HDAg) plays a critical role in virus replication. We previously demonstrated that the S-HDAg phospholylation occurs on both serine and threonine residues. However, their biological significance and the exact phosphorylation sites of S- HDAg are still unknown. In this study, phosphorylated S-HDAg was detected only in the intracellular compartment, not in viral particles. In addition , the number of phospholylated isoforms of S-HDAg significantly increased with the extent of viral replication in transfection system. Site-directed mutagenesis showed that alanine replacement of serine 177, which is conserved among all the known HDV strains, resulted in reduced phosphorylation of S-HDAg, while the mutation of the other two conserved serine residues (2 and 123) had little effect. The S177A mutant dramatically decreased its capability in assisting HDV RNA replication, with a preferential and profound impairment of the antigenomic RNA replication. Furthermore, the viral RNA editing, a step relying upon antigenomic RNA replication, was also abolished by this mutation. These results suggested that phosphorylation of S-HDAg, with serine 177 as a presumable site, plays a critical role in viral RNA replication, especially in augmenting the replication of antigenomic RNA. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | JOURNAL OF VIROLOGY v.75 n.19 pp.9087-9095 | en |
dc.relation.ispartof | JOURNAL OF | - |
dc.subject | PROTEIN-KINASE-C | en |
dc.subject | B SURFACE-ANTIGEN | en |
dc.subject | MOLECULAR-CLONING | en |
dc.subject | TRANSCRIPTION | en |
dc.subject | GENOME | en |
dc.subject | PHOSPHOPROTEIN | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | The Conserved Serine 177 in the Delta Antigen of Hepatitis Delta Virus Is One Putative Phosphorylation Site and Is Required for Efficient Viral Rna Replication | en |
dc.type | journal article | en |
dc.relation.pages | 9087-9095 | - |
dc.relation.journalvolume | VIROLOGY | - |
dc.relation.journalissue | n.19 | - |
item.languageiso639-1 | en_US | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | 醫學系 |
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