https://scholars.lib.ntu.edu.tw/handle/123456789/188270
標題: | T-Cell Antigenic Determinants within Hepatitis C Virus Nonstructural Protein 3 and Cytokine Production Profiles in Hepatitis C | 作者: | YANG, PEI-MING 陳培哲 |
關鍵字: | cytokines;hepatitis C virus;T-helper cell;NS3 PROTEIN;NON-A;INFECTION | 公開日期: | 2002 | 卷: | VIRAL | 期: | n.4 | 起(迄)頁: | 258-264 | 來源出版物: | JOURNAL OF | 摘要: | The aim of this study was to further investigate the role of T-helper cells in hepatitis C virus (HCV) infection, focusing on the T-cell antigenic determinants and cytokine profiles of nonstructural 3 (NS3) protein-stimulated peripheral blood mononuclear cells (PBMCs) of HCV patients. A total of 12 recombinant proteins of theNS3 region were purified and used to test T-cell proliferative response and antigenic determinants of HCV-seropositive patients. In addition, cytokines produced by antigen stimulated PBMCs were measured. Our data showed that PBMCs from 55.7% (34/61) of HCV patients proliferated to at least one antigen, but PBMCs of HCV seronegative patients did not. In addition, PBMCs from about 82.0% (32/39) HCV-seropositive patients produced significant amounts of cytokines (10 pg/mL). Interestingly, PBMCs from 66% of patients produced TH2 - related cytokines such as interleukin (IL)-4 and IL-5. In mappingexperiments, the data showed multiple T-cell antigenic determinants . Our data demonstrated that NS3 antigen-stimulated PBMCs of HCV patients recognized multiple T-cell antigenic determinants and produced significant amounts of TH0 or TH2 -related cytokines, which might play a critical role in the chronicity of HCV infection. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95218 |
顯示於: | 醫學系 |
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