https://scholars.lib.ntu.edu.tw/handle/123456789/188427
標題: | 17 Beta-Estradiol Inhibits Cyclic Strain-Induced Endothelin-1 Gene Expression within Vascular Endothelial Cells | 作者: | CHEN, JIN-JER CHENG, CHING-FENG CHAN, PAUL |
關鍵字: | strain;reactive oxygen species;extracellular signal- regulated kinase | 公開日期: | 2004 | 卷: | v.287 | 期: | n.3 | 起(迄)頁: | H1254-H1261 | 來源出版物: | AMERICAN JOURNAL OF PHYSIOLOGY. HEART AND CIRCULATORY PHYSIOLOGY | 摘要: | It has been well documented previously that 17beta-estradiol (E-2) exerts a protective effect on cardiovascular tissue. The possible role of E-2 in the regulation of endothelin (ET )-1 production has been previously reported, although the complex mechanisms by which E-2 inhibits ET-1 expression are not completely understood. The aims of this study were to examine whether E-2 was able to alter strain-induced ET-1 gene expression and also to identify the putative underlying signaling pathways that exist within endothelial cells. For cultured endothelial cells, E-2 ( 1 - 100 nM), but not 17 alpha-estradiol, inhibited the level of strain-induced ET- 1 gene expression and also peptide secretion. This inhibitory effect elicited by E-2 was able to be prevented by the coincubation of endothelial cells with the estrogen receptor antagonist ICI-182,780 ( 1 muM). E-2 also inhibited strain- enhanced NADPH oxidase activity and intracellular reactive oxygen species (ROS) generation as measured by the redox- sensitive fluorescent dye 2', 7'-dichlorofluorescin diacetate and the level of extracellular signal-regulated kinase (ERK) phosphorylation. Furthermore, the presence of E -2 and antioxidants such as N- acetylcysteine and diphenylene iodonium were able to elicit a decrease in the level of strain-induced ET-1 secretion, ET-1 promoter activity, ET-1 mRNA, ERK phosphorylation, and activator protein-1 binding activity. In summary, we demonstrated, for the first time, that E-2 inhibits strain- induced ET-1 gene expression, partially by interfering with the ERK pathway via the attenuation of strain-induced ROS generation. Thus this study delivers important new insight regarding the molecular pathways that may contribute to the proposed beneficial effects of estrogen on the cardiovascular system. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95279 |
顯示於: | 醫學系 |
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