https://scholars.lib.ntu.edu.tw/handle/123456789/188434
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 內科 | en |
dc.contributor.author | MU, JUNG-JUNG | en |
dc.contributor.author | TSAY, YEOU-GUANG | en |
dc.contributor.author | JUAN, LI-JUNG | en |
dc.contributor.author | CHEN, DING-SHINN | en |
dc.contributor.author | 陳培哲 | zh-tw |
dc.creator | 慕蓉蓉;蔡有光;阮麗蓉;陳定信;陳培哲 | zh-tw |
dc.creator | MU, JUNG-JUNG;TSAY, YEOU-GUANG;JUAN, LI-JUNG;CHEN, DING-SHINN;CHEN, PEI-JER | en |
dc.date | 2004 | en |
dc.date.accessioned | 2008-12-29T07:25:26Z | - |
dc.date.accessioned | 2018-07-11T05:34:47Z | - |
dc.date.available | 2008-12-29T07:25:26Z | - |
dc.date.available | 2018-07-11T05:34:47Z | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/95286 | - |
dc.description.abstract | Hepatitis delta virus (HDV) is a single-stranded RNA virus that encodes two viral nucleocapsid proteins named small and large form hepatitis delta antigen (S-HDAg and L-HDAg). The S-HDAg is essential for viral RNA replication while the L- HDAg is required for viral assembly. In this study, we demonstrated that HDAg are acetylated proteins. Metabolic labeling with [H-3]acetate revealed that both forms of HDAg could be acetylated in vivo. The histone acetyltransferase ( HAT) domain of cellular acetyltransferase p300 could acetylate the full-length and the N- terminal 88 amino acids of S-HDAg in vitro. By mass spectrometric analysis of the modified protein, Lys-72 of S-HDAg was identified as one of the acetylation sites. Substitution of Lys-72 to Arg caused the mutant S-HDAg to redistribute from the nucleus to the cytoplasm. The mutant reduced viral RNA accumulation and resulted in the earlier appearance of L-HDAg. These results demonstrated that HDAg is an acetylated protein and mutation of HDAg at Lys-72 modulates HDAg subcellular localization and may participate in viral RNA nucleocytoplasmic shuttling and replication. (C) 2003 Elsevier Inc. All rights reserved | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | VIROLOGY v.319 n.1 pp.60~70 | en |
dc.relation.ispartof | VIROLOGY | - |
dc.subject | HDV | en |
dc.subject | HDAg | en |
dc.subject | acetylation | en |
dc.subject | p300 | en |
dc.subject | NLS | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | The Small Delta Antigen of Hepatitis Delta Virus Is an Acetylated Protein and Acetylation of Lysine 72 May Influence Its Cellular Localization and Viral Rna Synthesis | en |
dc.type | journal article | en |
dc.relation.pages | 60-70 | - |
dc.relation.journalvolume | v.319 | - |
dc.relation.journalissue | n.1 | - |
item.languageiso639-1 | en_US | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
顯示於: | 醫學系 |
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