DC 欄位 | 值 | 語言 |
dc.contributor | 臺大醫院-內科部;臺大醫學院-腫瘤醫學研究所; | en |
dc.contributor.author | Yang, Tzu-Hsueh | en |
dc.contributor.author | Ou, Da-Liang | en |
dc.contributor.author | Hsu, Chiun | en |
dc.contributor.author | Huang, Shih-Hua | en |
dc.contributor.author | Chang, Po-Ling | en |
dc.creator | Yang, Tzu-Hsueh;Ou, Da-Liang;Hsu, Chiun;Huang, Shih-Hua;Chang, Po-Ling | en |
dc.creator | 歐大諒 | zh-tw |
dc.date | 2012 | en |
dc.date.accessioned | 2014-02-14T05:04:12Z | - |
dc.date.accessioned | 2018-07-11T06:45:53Z | - |
dc.date.available | 2014-02-14T05:04:12Z | - |
dc.date.available | 2018-07-11T06:45:53Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/259001 | - |
dc.description.abstract | A dual-LIF (dLIF) setup combined with CE for microRNA (miRNA) detection is proposed in this study. An argon ion laser (488 nm) and a solid state laser (640 nm) were chosen to excite the fluorescent dye-labeled DNA probe after splinted ligation of miRNA. The crosstalk of emission spectrum of Alex Fluor 488 and Alex Fluor 647 is minimized with a zero crosstalk matrix for Alex Fluor 647 to 488 channels. The linear ranges of the device for the fluorescent dye-labeled DNA probe were both from 1.0 nM to 0.1 pM. The limits of detection for Alexa Fluor 488-labeled DNA and Alex Fluor 647-labeled DNA were 9.3 and 31 fM, respectively. The detection of specific miRNA has been accomplished by combining splinted ligation with the fluorescent dye-labeled oligonucleotides. The linear range for the synthetic miRNA is from 1.0 nM to 1.0 pM. Without PCR amplification, CE-dLIF was applied to discriminate a pre-miR-10b*-transfected cells (contains precursor miR-10b*) from hepatocellular carcinoma cell (control cells). Therefore, this result indicates CE-dLIF has great potential to provide a rapid comparative assay for miRNAs detection. | en |
dc.format.extent | 104 bytes | - |
dc.format.mimetype | text/html | - |
dc.language | en-us | en |
dc.relation | Electrophoresis, 33(17), 2769-2776 | en |
dc.relation.ispartof | Electrophoresis | - |
dc.subject | Capillary electrophoresis | en |
dc.subject | Laser-induced fluorescence | en |
dc.subject | Poly(ethylene) oxide | en |
dc.subject | RNA | en |
dc.subject | Separation | en |
dc.title | Comparative microRNA detection from precursor-microRNA-transfected hepatocellular carcinoma cells by capillary electrophoresis with dual-color laser-induced fluorescence | en |
dc.relation.pages | 2769-2776 | - |
dc.relation.journalvolume | 33 | - |
dc.relation.journalissue | 17 | - |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/259001/1/index.html | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
顯示於: | 醫學系
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