https://scholars.lib.ntu.edu.tw/handle/123456789/191941
標題: | Effects of Omega-3 Fatty Acids on Leukocyte Th1l/Th2 Cytokine and Integrin Expression in Rats with Gut-Derived Sepsis | 作者: | LIN, MING-TSAN 李伯皇 |
關鍵字: | fish oil;gut-derived sepsis;immunoglobin A;lymphocyte subpopulation | 公開日期: | 2007 | 卷: | v.23 | 期: | n.2 | 起(迄)頁: | 179-186 | 來源出版物: | NUTRITION | 摘要: | Objectives: This study examined the effect of fish oil (FO)- enriched diets before and/or omega-3 fatty acid-containing total parenteral nutrition (TPN) after sepsis on the distribution of the T-lymphocyte subpopulation, intracellular cytokine, and intestinal immunity in rats with gut-derived sepsis. Methods: Rats were assigned to a control or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet, and groups 3 and 4 received FO instead of 20% soybean oil. After feeding the diets for 10 d, sepsis was induced by cecal ligation and puncture (CLP) in the experimental groups, whereas a sham operation was performed in the control group. TPN was maintained for 3 d after the CLP or sham operation. The control group and groups 1 and 3 were infused with conventional TPN, whereas the TPN solution used for groups 2 and 4 were supplemented with FO. All rats were sacrificed 3 d after the operation to examine their immune responses. Results: Plasma and intestinal immunoglobin A levels were higher in the FO-supplemented groups than in the control group and group 1. Lymphocyte interferon-gamma expression in groups 3 and 4 was significantly lower, whereas interteukin -4 expression was higher than those of the control group and groups 1 and 2. The splenocyte CD4 percentage in groups 3 and 4 and the CD4/CD8 ratio in cup 4 were significantly higher than those in group 1. Conclusion: These findings suggest that FO administration before and/or after CLP are not immunosuppressive. FO-enriched diets before or before and after CLP resulted in a T-helper type 2 response and enhanced immunoglobulin A secretion. In addition, the splenocyte CD4 levels and CD4/CD8 ratio were maintained in rats with gut-derived sepsis. (C) 2007 Elsevier Inc. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/91504 |
顯示於: | 醫學系 |
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