https://scholars.lib.ntu.edu.tw/handle/123456789/192327
標題: | E-cadherin 基因之promoter 區域(-160 C→A)多型性與大腸 直腸癌致癌危險性的關係 | 作者: | 梁金銅 | 關鍵字: | E-cadherin;基因多型性;黏著分子;大腸直腸癌;轉移;正位種植 | 公開日期: | 31-七月-2005 | 出版社: | 臺北市:國立臺灣大學醫學院外科 | 摘要: | 曾有報告指出E-cadherin 的promoter region 之-160℃→A 的基因多型性會增 減E-cadherin 基因的表現性。因此,評估E-cadherin 基因表現的多型性或許可以 鑑定出那些人具有罹患大腸直腸癌的危險性。然而,至今此類的報告卻只有零星 數篇而已。本研究是依個以台大醫院病例為基礎之病例控制性分析研究,我們共 收集了200 位大腸直腸癌病患與200 正常人。DNA 標本取自病患周邊血液白血 球,經聚合酶鏈鎖反應放大欲檢驗之DNA 片段,然後以限制酶切割分析基因多 型性。我們以回歸分析統計學計算此基因多型性的危險係數及95%信賴區間。並 經過統計調整其飲食生活習慣等因素(如嗜食紅肉、抽菸、喝酒、肥胖、糖尿病) 的影響。我們發現在200 位健康台灣人中,E-cadherin 基因型的分布是CC 佔42% (n=84),C/A 占48.0%(n=96),而AA 占10%(n=20)。而在大腸直腸癌病患 中AA 基因型的頻率(2%,n= 4 of 200)統計學上明顯比控制組的健康成人低 (p<0.005),其罹患大腸直腸癌的危險性約只有CC 基因型者的五分之ㄧ (OR=0.20;95% CI=0.06-0.56)。將這些大腸直腸癌患者依照病灶位置(左側vs. 右側大腸),組織學型態(分化良好、中度、以及不良),腫瘤期別(stage 0、Ⅰ 對stageⅡ、Ⅲ、Ⅳ期),以及淋巴腺轉移(陽性對陰性)等因子與E-cadherin 的 關聯分析中,並未發現任何相關。我們的結論E-cadherin-160A/A 基因型會降低 大腸直腸癌罹病的危險性。接下來必須探討A 型態的基因型到底在腫瘤組織中對 E-cadherin 功能所產生的影響,以及收集更多的病例,以進一步確定相關性。 A 160℃→A polymorphism in the promoter region of E-cadherin has been reported to decrease gene transcription. This allelic variation might be a potential genetic marker for identifying individuals at risk for colorectal cancer. Up to date, only very few reports were published the regarding clarification of the association between the polymorphism of E-cadherim and colorectal cancer. A hospital-based case-control study, including 200 colorectal cancer patients and 200 unaffected controls, was performed. DNA from peripheral blood samples was examined by polymerase chain reaction-restriction fragment length polymorphism. Logistic regression analyses were used to computed odds ratio (OR) and 95% confidence interval (CI) after adjusted for the influence from diet and life-style factors (e.g. red meat, smoking, drinking, obesity, and diabetes mellitus ). In 200 healthy Taiwanese, the distribution of genotype C/C was 42% (n=84), C/A was 48.0% (n=96), and AA was 10% (n=20). The frequency of variant A/A genotype in colorectal cancer cases (4 of 200, 2%) was significantly lower than that of controls (20 of 200, 10%) (p<0.005), conferring a 5-hold decrease in the risk of colorectal cancer (OR, 0.20 : 95% CI, 0.06-0.56) compared with the C/C genotype. Stratification of the colorectal cancer cases according to their location (left-sided vs. right-sided colon), histology (well, moderate, and poor differentiation), tumor stage (stage 0, Ⅰvs. stageⅡ,Ⅲ,Ⅳ), and lymph node metastasis(positive and negative) fail to reveal any heterogeneity with resped to E-cadherin genotype. The present data suggest that individuals with E-cadherin-160 A/A genotype have a decreased risk of colorectal cancer. Further study is mandatory to clarify the functional relevance of the A allele in vivo and to confirm the inverse association of the A/A genotype with colorectal cancer in large epidemiologic studies. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/24557 | 其他識別: | 932314B002259 | Rights: | 國立臺灣大學醫學院外科 |
顯示於: | 醫學系 |
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932314B002259.pdf | 493.13 kB | Adobe PDF | 檢視/開啟 |
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