https://scholars.lib.ntu.edu.tw/handle/123456789/192328
標題: | 結締組織生長因子在大腸直腸癌的侵犯和轉移的角色扮演 Connective Tissue Growth Factor Inhibits Metastasis and Acts as an Independent Prognostic Marker in Colorectal Cancer |
作者: | 林本仁 Lin, Been-Ren |
公開日期: | 31-七月-2005 | 出版社: | 臺北市:國立臺灣大學醫學院外科 | 起(迄)頁: | 9-23 | 來源出版物: | GASTROENTEROLOGY 128(1) | 摘要: | Connective tissue growth factor (CTGF) has been shown to be implicated in tumor development and progression. The aim of this study was to investigate the role of CTGF in progression of colorectal cancer (CRC). Methods: Immunohistochemical staining of specimens from 119 patients with CRC was performed. Liposome-mediated transfection was used to introduce a CTGF expression vector into CRC cell lines. Transfectants were tested in invasive ability and experimental hepatic metastasis in BALB/c mice. Furthermore, a FOPflash/TOPflash reporter assay was performed to investigate CTGF on the -catenin/T-cell factor signaling pathway. Results: Patients with stage II and stage III CRC whose tumors displayed high CTGF expression had a significantly higher overall survival and a disease-free advantage over patients with CRC with low CTGF expression. Alterations in the CTGF level in CRC cell lines modulated their invasive ability with an inverse correlation. In addition, a reduction in the CTGF level of CT26 cells after stable transfection with antisense CTGF resulted in increased liver metastasis in BALB/c mice. The activity of the -catenin/T-cell factor signaling pathway and its downstream effector gene matrix metalloproteinase 7 in these CTGF-transfected cells was strongly attenuated. Blockage of matrix metalloproteinase 7 with its neutralizing antibodies inhibited increased invasiveness in antisense CTGF-transfected CT26 cells. Conclusions: Our results implicate CTGF as a key regulator of CRC invasion and metastasis, and it appears to be a useful and better prognosis factor for patients with stage II and stage III CRC. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/24558 | 其他識別: | 932314B002261 | Rights: | 國立臺灣大學醫學院外科 |
顯示於: | 醫學系 |
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