|Title:||Reappraisal of K-Ras and P53 Gene Mutations in the Recurrence of Dukes' B 2 Rectal Cancer after Curative Resection||Authors:||LIANG, JIN-TUNG
|Keywords:||p53;K-ras;rectal cancer;recurrence;COLORECTAL-CANCER;CARCINOMA||Issue Date:||1999||Journal Volume:||v.46||Journal Issue:||n.26||Start page/Pages:||830-837||Source:||HEPATO-GASTROENTEROLOGY||Abstract:||
BACKGROUND/AIMS: Recurrence of rectal cancer remains a major clinical problem. This study was conducted to evaluate the impact of K-ras and p53 mutations on the recurrence of rectal cancer. METHODOLOGY: A total of 166 resected Dukes' B 2 stage rectal carcinomas were collected between January 1990 and April 1994. The stored frozen tissues were retrieved for immunocytochemistry of p53 and genomic analysis of K-ras and p53 genes. The data of K-ras and p53 gene mutations were correlated with clinicopathological variables. The concordance of immunocytochemistry with genomic analysis in the survey of p53-mutations was examined . The follow -up data were analyzed by Kaplan-Meier estimator . RESULTS: Sixty-nine patients (41.6%) developed recurrent tumor. A significantly higher recurrence rate (p=0.0013) and shorter median recurrence time were noted in p53 mutated than non-mutated cancers. Mutations in K-ras gene do not significantly increase the risk of tumor recurrence (p=0. 1702), K-ras and p53 mutations are not associated with clinicopathological parameters (p>0 .05). Kappa statistic indicates highly significant reproducibility between immunocytochemistry and genomic analysis for p53 mutations ( p<0.0001). CONCLUSIONS: Presence of p53 mutation significantly increases the recurrence rate and shortens the recurrence-time of the resected rectal cancers. Pre- operative routine check for p53 mutations by immunocytochemistry may be beneficial in choosing the optimal surgical strategy for rectal cancer.
|Appears in Collections:||醫學系|
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