https://scholars.lib.ntu.edu.tw/handle/123456789/193181
標題: | Dephosphorylation of Cancer Protein by Tyrosine Phosphatases in Response to Analogs of Luteinizing Hormone-Releasing Hormone and Somatostatin | 作者: | LEE, LUNG-TA LEE, PO-HUANG LEE, MING- TING 李伯皇 |
關鍵字: | LHRH;somatostatin;protein tyrosine kinase;protein tyrosin phosphatase;receptor | 公開日期: | 2008 | 卷: | v.28 | 期: | n.5A | 起(迄)頁: | 2599-2605 | 來源出版物: | ANTICANCER RESEARCH | 摘要: | Protein phosphorylation/dephosphorylation of tyrosine residues is an important regulatory mechanism in cell growth and differentiation. Previously it has been reported that RC-160, an octapeptide analog of somatostatin, and [D-Trp(6) ]LHRH, an agonist of luteinizing hormone- releasing hormone ( LHRH), stimulate receptor-mediated activity of tyrosine phosphatases (PTP) and reverse growth promotion of the tyrosine kinase (PTK) class of oncogenes in tumor cells. The effect of RC-160 and [ D-Trp(6)]LHRH on protein phosphorylation was further examined in surgical specimens of human carcinomas. Protein extracts of human ovarian, liver, breast and prostate tumor samples were preincubated with epidermal growth factor (EGF) (10(-7) M) with or without [D-Trp(6)]LHRH or RC-160 (10(-6) M ) at 25 degrees C for 2 h, followed by incubation for 10 min with [gamma-( 32)p ]ATp. SDS-PAGE, Western blotting, autoradiography and densitometry were then used to quantify the phosphorylation level of individual protein bands. It was found that EGF enhanced, and [D-Trp(6)]LHRH and RC-160 reduced phosphorylation of a prominent 300-kDa band. Two proteins ( 65 and 60 kDa), involved in growth control in tumor cell lines, were also identified in this study. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/174636 |
顯示於: | 醫學系 |
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